Microfluidic cytometric analysis of cancer cell transportability and invasiveness

被引:43
作者
Liu, Zongbin [1 ,2 ]
Lee, Yeonju [1 ]
Jang, Joon Hee [1 ,2 ]
Li, Ying [1 ,2 ]
Han, Xin [1 ,2 ]
Yokoi, Kenji [1 ]
Ferrari, Mauro [1 ,3 ]
Zhou, Ledu [1 ,2 ,4 ]
Qin, Lidong [1 ,2 ,5 ]
机构
[1] Houston Methodist Res Inst, Dept Nanomed, Houston, TX 77030 USA
[2] Cornell Univ, Weill Med Coll, Dept Cell & Dev Biol, New York, NY 10065 USA
[3] Weill Cornell Med Coll, Dept Med, New York, NY 10065 USA
[4] Cent South Univ, Xiangya Hosp, Dept Gen Surg, Changsha 410008, Hunan, Peoples R China
[5] Univ Texas MD Anderson Canc Ctr, Dept Mol & Cellular Oncol, Houston, TX 77030 USA
来源
SCIENTIFIC REPORTS | 2015年 / 5卷
关键词
INFLAMMATORY BREAST-CANCER; TUMOR-CELLS; DEFORMABILITY; PROGRESSION; SEPARATION; ADHESION; INVASION; ALPHA; EMT;
D O I
10.1038/srep14272
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The extensive phenotypic and functional heterogeneity of cancer cells plays an important role in tumor progression and therapeutic resistance. Characterizing this heterogeneity and identifying invasive phenotype may provide possibility to improve chemotherapy treatment. By mimicking cancer cell perfusion through circulatory system in metastasis, we develop a unique microfluidic cytometry (MC) platform to separate cancer cells at high throughput, and further derive a physical parameter 'transportability' to characterize the ability to pass through micro-constrictions. The transportability is determined by cell stiffness and cell-surface frictional property, and can be used to probe tumor heterogeneity, discriminate more invasive phenotypes and correlate with biomarker expressions in breast cancer cells. Decreased cell stiffness and cell-surface frictional force leads to an increase in transportability and may be a feature of invasive cancer cells by promoting cell perfusion through narrow spaces in circulatory system. The MC-Chip provides a promising microfluidic platform for studying cell mechanics and transportability could be used as a novel marker for probing tumor heterogeneity and determining invasive phenotypes.
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收藏
页数:12
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