The role of the CD4 receptor versus HIV coreceptors in envelope-mediated apoptosis in peripheral blood mononuclear cells

被引:30
作者
Arthos, J [1 ]
Cicala, C [1 ]
Selig, SM [1 ]
White, AA [1 ]
Ravindranath, HM [1 ]
Van Ryk, D [1 ]
Steenbeke, TD [1 ]
Machado, E [1 ]
Khazanie, P [1 ]
Hanback, MS [1 ]
Hanback, DB [1 ]
Rabin, RL [1 ]
Fauci, AS [1 ]
机构
[1] Natl Inst Hlth, NIAID, Immunoregulat Lab, Bethesda, MD 20892 USA
关键词
CCR5; CXCR4; retrovirus; gp120; TUNEL; annexin-V; T cell;
D O I
10.1006/viro.2001.1266
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We examined the role of CD4, CXCR4, and CCR5 in HIV envelope-mediated apoptosis by measuring the response of activated PBMCs to recombinant envelope proteins derived from CXCR4- and CCR5-utilizing viruses. Apoptosis of T cells was assessed by annexin-V staining and TdT-mediated dUTP-biotin nick-end labeling. Treatment of CCR5Delta32 homozygote PBMCs with a CCR5-specific envelope induced apoptosis in T cells, demonstrating that envelope-CD4 interactions are sufficient to induce apoptosis. However, a CXCR4-specific envelope induced higher levels of apoptosis than a CCR5-specific envelope, suggesting that envelope-mediated apoptosis can be enhanced by envelope-CXCR4 interactions. We conclude that envelope can induce apoptosis in T cells independently of the coreceptor specificity of a given envelope, or the expression profile of CXCR4 or CCR5 on a target cell. However, envelope-coreceptor interactions, and in particular, envelope-CXCR4 interactions, can contribute to this process. (C) 2002 Elsevier Science.
引用
收藏
页码:98 / 106
页数:9
相关论文
共 53 条
[1]   PRODUCTION OF ACQUIRED IMMUNODEFICIENCY SYNDROME-ASSOCIATED RETROVIRUS IN HUMAN AND NONHUMAN CELLS TRANSFECTED WITH AN INFECTIOUS MOLECULAR CLONE [J].
ADACHI, A ;
GENDELMAN, HE ;
KOENIG, S ;
FOLKS, T ;
WILLEY, R ;
RABSON, A ;
MARTIN, MA .
JOURNAL OF VIROLOGY, 1986, 59 (02) :284-291
[2]   CCR5 signal transduction in macrophages by human immunodeficiency virus and simian immunodeficiency virus envelopes [J].
Arthos, J ;
Rubbert, A ;
Rabin, RL ;
Cicala, C ;
Machado, E ;
Wildt, K ;
Hanbach, M ;
Steenbeke, TD ;
Swofford, R ;
Farber, JM ;
Fauci, AS .
JOURNAL OF VIROLOGY, 2000, 74 (14) :6418-6424
[3]   RANTES induces tyrosine kinase activity of stably complexed p125(FAK) and ZAP-70 in human T cells [J].
Bacon, KB ;
Szabo, MC ;
Yssel, H ;
Bolen, JB ;
Schall, TJ .
JOURNAL OF EXPERIMENTAL MEDICINE, 1996, 184 (03) :873-882
[4]  
Badley A D, 1997, Adv Pharmacol, V41, P271, DOI 10.1016/S1054-3589(08)61062-5
[5]   CXCR4 and CD4 mediate a rapid CD95-independent cell death in CD4+ T cells [J].
Berndt, C ;
Möpps, B ;
Angermüller, S ;
Gierschik, P ;
Krammer, PH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (21) :12556-12561
[6]   Caspase-dependent apoptosis of cells expressing the chemokine receptor CXCR4 is induced by cell membrane-associated human immunodeficiency virus type 1 envelope glycoprotein (gp120) [J].
Biard-Piechaczyk, M ;
Robert-Hebmann, V ;
Richard, V ;
Roland, J ;
Hipskind, RA ;
Devaux, C .
VIROLOGY, 2000, 268 (02) :329-344
[7]  
BIBERFELD P, 1986, AM J PATHOL, V125, P436
[8]   The implication of the chemokine receptor CXCR4 in HIV-1 envelope protein-induced apoptosis is independent of the G protein-mediated signalling [J].
Blanco, J ;
Jacotot, E ;
Cabrera, C ;
Cardona, A ;
Clotet, B ;
De Clercq, E ;
Esté, JA .
AIDS, 1999, 13 (08) :909-917
[9]   MEASUREMENT OF APOPTOTIC CELLS IN PERIPHERAL-BLOOD [J].
CARBONARI, M ;
CIBATI, M ;
FIORILLI, M .
CYTOMETRY, 1995, 22 (03) :161-167
[10]   BIOLOGIC FEATURES OF HIV-1 THAT CORRELATE WITH VIRULENCE IN THE HOST [J].
CHENGMAYER, C ;
SETO, D ;
TATENO, M ;
LEVY, JA .
SCIENCE, 1988, 240 (4848) :80-82