Optical needle endoscope for safe and precise stereotactically guided biopsy sampling in neurosurgery

被引：28

作者：

Goebel, Werner ^{[1
]}

Brucker, David ^{[2
]}

Kienast, Yvonne ^{[2
]}

Johansson, Ann ^{[3
]}

Kniebuehler, Gesa ^{[3
]}

Ruehm, Adrian ^{[3
]}

Eigenbrod, Sabina ^{[2
]}

Fischer, Stefan ^{[4
]}

Goetz, Marcus ^{[4
]}

Kreth, Friedrich-Wilhelm ^{[5
]}

Ehrhardt, Andre ^{[1
]}

Stepp, Herbert ^{[3
]}

Irion, Klaus-Martin ^{[1
]}

Herms, Jochen ^{[2
,6
]}

机构：

[1] KARL STORZ GmbH & Co KG, Dept Res & Technol, D-78532 Tuttlingen, Germany

[2] Univ Munich, Dept Neuropathol, D-81377 Munich, Germany

[3] Univ Munich, Laser Res Lab, D-81377 Munich, Germany

[4] MRC Syst GmbH, D-69123 Heidelberg, Germany

[5] Univ Munich, Dept Neurosurg, D-81377 Munich, Germany

[6] German Ctr Neurodegenerat Dis eV DZNE, Dept Translat Neurodegenerat 1, D-81377 Munich, Germany

来源：

OPTICS EXPRESS | 2012年 / 20卷 / 24期

关键词：

FLUORESCENCE; MICROSCOPY; REVEALS; TISSUE; TUMORS;

D O I：

10.1364/OE.20.026117

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Proper treatment of deep seated brain tumors requires correct histological diagnosis which unambiguously necessitates biopsy sampling. Stereotactically guided sampling of biopsies is widely used but bears the danger of incorrect sampling locations and damage to intracerebral blood vessels. Here, we present a minimally invasive contact endoscopic probe that can be inserted into the tissue inside a standard biopsy needle and allows for fluorescence detection of both tumorous tissue and intracerebral blood vessels. Outer diameter of our contact probe is smaller than 1.5 mm, field-of-view in the range of several hundred microns; the optical design allows for simultaneous detection and visualization of tissue autofluorescence and selective fluorescence signals from deep seated brain tumors and vasculature as shown on in vivo animal models. We demonstrate the tumor detection capability during stereotactic needle insertion in a clinical pilot trial. Using our probe, we expect stereotactic interventions to become safer and more precise and the technology might ultimately be used also for various other kinds of applications. (c) 2012 Optical Society of America

引用

页码：26117 / 26126

页数：10

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