The PqqD homologous domain of the radical SAM enzyme ThnB is required for thioether bond formation during thurincin H maturation

被引：63

作者：

Wieckowski, Beata M. ^{[1
]}

Hegemann, Julian D. ^{[1
]}

Mielcarek, Andreas ^{[1
]}

Boss, Linda ^{[1
]}

Burghaus, Olaf ^{[1
]}

Marahiel, Mohamed A. ^{[1
]}

机构：

[1] Univ Marburg, Dept Chem Biochem, LOEWE Ctr Synthet Microbiol, D-35032 Marburg, Germany

来源：

FEBS LETTERS | 2015年 / 589卷 / 15期

关键词：

Natural product; Ribosomal peptide; Sactipeptide; Biosynthesis; Radical SAM enzyme; 4Fe-4S] cluster; INDEPENDENT-MUTAGENESIS SLIM; BIOTIN SYNTHASE; SUBTILOSIN-A; THURICIN CD; SULFUR; BACTERIOCIN; PEPTIDE;

D O I：

10.1016/j.febslet.2015.05.032

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thurincin H is a 31-residue, ribosomally synthesized bacteriocin originating from the thn operon of Bacillus thuringiensis SF361. It is the only known sactipeptide carrying four thioether bridges between four cysteines and the alpha-carbons of a serine, an asparagine and two threonine residues. By analysis of the thn operon and use of in vitro studies we now reveal that ThnB is a radical S-adenosylmethionine (SAM) enzyme containing two [4Fe-4S] clusters. Furthermore, we confirm the involvement of ThnB in the formation of the thioether bonds present within the structure of thurincin H. Finally, we show that the PqqD homologous N-terminal domain of ThnB is essential for maturation of the thurincin H precursor peptide, but not for the SAM cleavage activity of ThnB. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：1802 / 1806

页数：5

共 27 条

[1] Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature [J].

Arnison, Paul G. ;

Bibb, Mervyn J. ;

Bierbaum, Gabriele ;

Bowers, Albert A. ;

Bugni, Tim S. ;

Bulaj, Grzegorz ;

Camarero, Julio A. ;

Campopiano, Dominic J. ;

Challis, Gregory L. ;

Clardy, Jon ;

Cotter, Paul D. ;

Craik, David J. ;

Dawson, Michael ;

Dittmann, Elke ;

Donadio, Stefano ;

Dorrestein, Pieter C. ;

Entian, Karl-Dieter ;

Fischbach, Michael A. ;

Garavelli, John S. ;

Goeransson, Ulf ;

Gruber, Christian W. ;

Haft, Daniel H. ;

Hemscheidt, Thomas K. ;

Hertweck, Christian ;

Hill, Colin ;

Horswill, Alexander R. ;

Jaspars, Marcel ;

Kelly, Wendy L. ;

Klinman, Judith P. ;

Kuipers, Oscar P. ;

Link, A. James ;

Liu, Wen ;

Marahiel, Mohamed A. ;

Mitchell, Douglas A. ;

Moll, Gert N. ;

Moore, Bradley S. ;

Mueller, Rolf ;

Nair, Satish K. ;

Nes, Ingolf F. ;

Norris, Gillian E. ;

Olivera, Baldomero M. ;

Onaka, Hiroyasu ;

Patchett, Mark L. ;

Piel, Joern ;

Reaney, Martin J. T. ;

Rebuffat, Sylvie ;

Ross, R. Paul ;

Sahl, Hans-Georg ;

Schmidt, Eric W. ;

Selsted, Michael E. .

NATURAL PRODUCT REPORTS, 2013, 30 (01) :108-160

[2] SUBTILOSIN-A, A NEW ANTIBIOTIC PEPTIDE PRODUCED BY BACILLUS-SUBTILIS-168 - ISOLATION, STRUCTURAL-ANALYSIS, AND BIOGENESIS [J].

BABASAKI, K ;

TAKAO, T ;

SHIMONISHI, Y ;

KURAHASHI, K .

JOURNAL OF BIOCHEMISTRY, 1985, 98 (03) :585-603

[3] Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h [J].

Chiu, J ;

March, PE ;

Lee, R ;

Tillett, D .

NUCLEIC ACIDS RESEARCH, 2004, 32 (21) :e174

[4] Site-directed, Ligase-Independent Mutagenesis (SLIM) for highly efficient mutagenesis of plasmids greater than 8kb [J].

Chiu, Joyce ;

Tillett, Daniel ;

Dawes, Ian W. ;

March, Paul E. .

JOURNAL OF MICROBIOLOGICAL METHODS, 2008, 73 (02) :195-198

[5] Control of radical chemistry in the AdoMet radical enzymes [J].

Duschene, Kaitlin S. ;

Veneziano, Susan E. ;

Silver, Sunshine C. ;

Broderick, Joan B. .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2009, 13 (01) :74-83

[6]

Flühe E, 2012, NAT CHEM BIOL, V8, P350, DOI [10.1038/NCHEMBIO.798, 10.1038/nchembio.798]

[7] Radical S-adenosylmethionine enzyme catalyzed thioether bond formation in sactipeptide biosynthesis [J].

Fluehe, Leif ;

Marahiel, Mohamed A. .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2013, 17 (04) :605-612

[8] Two [4Fe-4S] Clusters Containing Radical SAM Enzyme SkfB Catalyze Thioether Bond Formation during the Maturation of the Sporulation Killing Factor [J].

Fluehe, Leif ;

Burghaus, Olaf ;

Wieckowski, Beata M. ;

Giessen, Tobias W. ;

Linne, Uwe ;

Marahiel, Mohamed A. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2013, 135 (03) :959-962

[9]

Frey PA, 2008, CRIT REV BIOCHEM MOL, V43, P63, DOI [10.1080/10409230701829169, 10.1080/10409230701829169 ]

[10] Biotin synthase: Insights into radical-mediated carbon-sulfur bond formation [J].

Fugate, Corey J. ;

Jarrett, Joseph T. .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2012, 1824 (11) :1213-1222

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