Differential Expression Analysis of Diabetic Related Genes in Streptozotocin-Induced Diabetic Rat in Response to Abelmoschus Esculentus Treatment:A Research Framework

Diabetes is characterized by excessive blood sugar due to body's failure to produce insulin or the consequence of insulin resistance. Nowadays, human suffering not only on the disease itself but also includes diabetes-related complications. All these abnormality are significantly important because of their effects on the liver function. Therefore this paper will provides an overview of the research framework to investigate the differential expression analysis of liver tissue in streptozotocin-induced diabetic rat in response to Abelmoschus esculentus (AE) treatment. The hypoglycemic effects of water extract prepared from the fruit of AE will be studied in diabetic rats (streptozotocin induced). All animals will be randomly divided into three group; normal healthy group (N group), streptozotocin-induced diabetic group (STZ group) and AE-treated diabetic group (AE group). Oral application of AE at doses of 100, 150 and 200 mg/kg body weight will be given to AE-treated diabetic group by single and repeated oral administration. Rats liver from these three groups will then subjected to RNA extraction for gene expression analysis. The differential expression study will be carried out by using real time RT-PCR method. Four diabetes-specific genes of interest (carboxylesterase 2, stearoyl-Coenzyme A desaturase 1, insulin-like growth factor 1 and insulin-like growth factor binding protein 2 binding protein) are chosen and the expression level of these genes will be examined quantitatively. The abnormal expression of genes in STZ group will be rescued by the AE therapy. The expected findings from both in vivo and molecular studies may reveal the anti-diabetic properties of the AE and suggest that the plant extract may be useful for the management of the disease. This paper will provide the overview of the research framework and giving insight of the experimental procedure to be implemented.