Synthesis, characterization, and properties of novel hydrophobically associating fluorinated copolymers for DNA delivery

被引：7
作者：
Kuang, Wei [1 ,2 ]
Zhao, Xiaodong [3 ]
机构：
[1] Shandong Polytech Univ, Sch Chem & Pharmaceut Engn, Jinan 250353, Peoples R China
[2] Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Shandong, Peoples R China
[3] Zhongpi Tianyuan Sci & Technol Co Ltd, China Leather & Footwear Ind Res Inst, Beijing 100015, Peoples R China
来源：
REACTIVE & FUNCTIONAL POLYMERS | 2013年 / 73卷 / 05期
关键词：
Hydrophobic association; Block copolymer; Fluorocarbon; Gene vector; GENE DELIVERY; MICELLIZATION KINETICS; DIBLOCK COPOLYMER; BLOCK-COPOLYMERS; COMPLEXES; POLYMERS; THERAPY; SYSTEMS;
D O I：
10.1016/j.reactfunctpolym.2013.02.010
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Novel fluorinated associative copolymers poly(ethylene glycol)-b-poly(2-(perfluorohexyl)ethyl methacrylate (PFHEMA)-co-3-[N-(2-methacroyloylethyl)-N,N-dimethylammonio]-propane sulfonate (DMAPS)) (PEG-b-PPFHEMA/PDMAPS) were prepared as a type of non-viral gene vector. The series of PEG-b-PFHEMA/DMAPS with different molecular weights and compositions were characterized by gel permeation chromatography-multi-angle light scattering, H-1 NMR, F-19 NMR, and elemental analysis. The interactions of the copolymers with calf thymus DNA and the morphologies of the resulting complexes were studied by fluorescence spectroscopy, dynamic light scattering, and transmission electron microscopy. In addition, the DNA-polymer interaction, as determined by the gel retardation assay, and the low cytotoxicity of PEG-b-PPFHEMA/PDMAPS demonstrated that these copolymers have good DNA binding capacity and potential for high performance in the practical application of DNA delivery. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：703 / 709
页数：7
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