Photonic crystal surface mode imaging for multiplexed and high-throughput label-free biosensing

被引：15

作者：

Konopsky, Valery ^{[1
]}

Mitko, Tatiana ^{[2
]}

Aldarov, Konstantin ^{[2
]}

Alieva, Elena ^{[1
]}

Basmanov, Dmitry ^{[2
]}

Moskalets, Aleksandr ^{[2
]}

Matveeva, Ainur ^{[2
]}

Morozova, Olga ^{[2
]}

Klinov, Dmitry ^{[2
]}

机构：

[1] Inst Spect, Fizicheskaya 5, Troitsk 108840, Russia

[2] Fed Med Biol Agcy, Fed Res & Clin Ctr Phys Chem Med, Pirogovskaya 1a, Moscow 119435, Russia

来源：

BIOSENSORS & BIOELECTRONICS | 2020年 / 168卷

基金：

俄罗斯科学基金会; 俄罗斯基础研究基金会;

关键词：

Label-free optical biosensors; Surface wave imaging; Photonic crystal surface modes; SPRi; PCSMi; PLASMON RESONANCE; WAVES;

D O I：

10.1016/j.bios.2020.112575

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A photonic crystal surface mode imaging (PCSMi) technique is implemented for the simultaneous detection of antibody binding with specific antigens in arrays containing 96- and 384-spots. Like the surface plasmon resonance imaging (SPRi) technique, the presented approach is label-free and permits interrogating an analyte by hundreds of different ligands immobilized in small spots. The adsorption kinetics is recorded with a subpicogram resolution at every spot simultaneously. Possible implementations of this technique for multiplexed and high-throughput biosensing are discussed.

引用

页数：5