Long non-coding RNA MEG3 silencing protects against light-induced retinal degeneration

被引：33
作者：
Zhu, Yun-Xi [1 ,2 ]
Yao, Jin [1 ,2 ]
Liu, Chang [2 ,3 ]
Hu, Hai-Tao [1 ,2 ]
Li, Xiu-Miao [1 ]
Ge, Hui-Min [2 ]
Zhou, Yun-Fan [2 ]
Shan, Kun [3 ]
Jiang, Qin [1 ,2 ]
Yan, Biao [3 ,4 ]
机构：
[1] Nanjing Med Univ, Eye Hosp, Nanjing, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Sch Clin Med 4, Nanjing, Jiangsu, Peoples R China
[3] Fudan Univ, Shanghai Med Coll, Eye & ENT Hosp, Eye Inst, Shanghai, Peoples R China
[4] Shanghai Key Lab Visual Impairment & Restorat, Shanghai, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2018年 / 496卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Long non-coding RNA; Light insult; Retinal degeneration; Protein decoy; OXIDATIVE STRESS; MICROVASCULAR DYSFUNCTION; MOLECULAR-MECHANISMS; DOCOSAHEXAENOIC ACID; CELL-PROLIFERATION; TUMOR-SUPPRESSOR; GENE-EXPRESSION; IN-VITRO; APOPTOSIS; P53;
D O I：
10.1016/j.bbrc.2018.01.177
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Excessive light exposure leads to retinal degeneration and accelerates the progression and severity of several ocular diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa. Long non-coding RNAs (LncRNAs) have emerged as important regulators of photoreceptor development and ocular diseases. In this study, we investigated the role of IncRNA-MEG3 in light-induced retinal degeneration. MEG3 expression was significantly up-regulated after light insult in vivo and in vitro. MEG3 silencing protected against light-induced retinal degeneration in vivo and light-induced photoreceptor cell apoptosis in vitro. Mechanistically, MEG3 regulated retinal photoreceptor cell function by acting as p53 decoy. MEG3 silencing decreased caspase 3/7 activity, up-regulated anti-apoptotic protein (Bcl-2) expression, and down-regulated pro-apoptotic protein (Bax) expression. Taken together, this study provides a promising method of MEG3 silencing for treating light-induced retinal degeneration. (C) 2018 Elsevier Inc. All rights reserved.
引用
收藏
页码：1236 / 1242
页数：7
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