In vivo Wnt signaling tracing through a transgenic biosensor fish reveals novel activity domains

被引：192

作者：

Moro, Enrico ^{[1
]}

Ozhan-Kizil, Gunes ^{[2
]}

Mongera, Alessandro ^{[3
]}

Beis, Dimitris ^{[4
]}

Wierzbicki, Claudia ^{[5
]}

Young, Rodrigo M. ^{[5
]}

Bournele, Despina ^{[4
]}

Domenichini, Alice ^{[6
]}

Valdivia, Leonardo E. ^{[5
]}

Lum, Lawrence ^{[7
]}

Chen, Chuo ^{[8
]}

Amatruda, James F. ^{[9
,10
,11
]}

Tiso, Natascia ^{[6
]}

Weidinger, Gilbert ^{[2
]}

Argenton, Francesco ^{[6
]}

机构：

[1] Univ Padua, Dept Biomed Sci, I-35121 Padua, Italy

[2] Tech Univ Dresden, Ctr Biotechnol, D-01307 Dresden, Germany

[3] Max Planck Inst Dev Biol, D-72076 Tubingen, Germany

[4] Biomed Res Fdn Acad Athens, Athens, Greece

[5] UCL, Dept Cell & Dev Biol, London WC1E 6BT, England

[6] Univ Padua, Dept Biol, I-35121 Padua, Italy

[7] Univ Texas SW Med Ctr Dallas, Dept Cell Biol, Dallas, TX 75390 USA

[8] Univ Texas SW Med Ctr Dallas, Dept Biochem, Dallas, TX 75390 USA

[9] Univ Texas SW Med Ctr Dallas, Dept Pediat, Dallas, TX 75390 USA

[10] Univ Texas SW Med Ctr Dallas, Dept Internal Med, Dallas, TX 75390 USA

[11] Univ Texas SW Med Ctr Dallas, Dept Mol Biol, Dallas, TX 75390 USA

来源：

DEVELOPMENTAL BIOLOGY | 2012年 / 366卷 / 02期

关键词：

Zebrafish; Wnt/beta-catenin; Reporter; Confocal microscopy; Small-chemicals; Biosensor; WNT/BETA-CATENIN; BETA-CATENIN; STEM-CELLS; AORTIC ENDOTHELIUM; ZEBRAFISH; TRANSCRIPTION; GENES; ROLES; LEF1; DIFFERENTIATION;

D O I：

10.1016/j.ydbio.2012.03.023

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The creation of molecular tools able to unravel in vivo spatiotemporal activation of specific cell signaling events during cell migration, differentiation and morphogenesis is of great relevance to developmental cell biology. Here, we describe the generation, validation and applications of two transgenic reporter lines for Wnt/beta-catenin signaling, named TCFsiam, and show that they are reliable and sensitive Wnt biosensors for in vivo studies. We demonstrate that these lines sensitively detect Wnt/beta-catenin pathway activity in several cellular contexts, from sensory organs to cardiac valve patterning. We provide evidence that Wnt/beta-catenin activity is involved in the formation and maintenance of the zebrafish CNS blood vessel network, on which sox10 neural crest-derived cells migrate and proliferate. We finally show that these transgenic lines allow for screening of Wnt signaling modifying compounds, tissue regeneration assessment as well as evaluation of potential Wnt/beta-catenin genetic modulators. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：327 / 340

页数：14

共 71 条

[11] Armadillo/β-catenin signals in the nucleus -: proof beyond a reasonable doubt? [J].