Immunogenicity of engineered probiotics expressing conserved antigens of influenza virus and FLIC flagellin against H9N2 AIVinfection in mice

被引：2

作者：

Hu, Tian-Yang ^{[1
]}

Lian, Yi-Bing ^{[1
]}

Qian, Jia-Hao ^{[1
]}

Yang, Yong-Lei ^{[1
]}

Ata, Emad Beshir ^{[2
]}

Zhang, Rong-Rong ^{[1
]}

Shi, Chun-Wei ^{[1
]}

Yang, Gui-Lian ^{[1
]}

Huang, Hai-Bin ^{[1
]}

Jiang, Yan-Long ^{[1
]}

Wang, Jian-Zhong ^{[1
]}

Cao, Xin ^{[1
]}

Wang, Nan ^{[1
]}

Zeng, Yan ^{[1
]}

Yang, Wen-Tao ^{[1
]}

Wang, Chun-Feng ^{[1
]}

机构：

[1] Jilin Agr Univ, Jilin Prov Engn Res Ctr Anim Probiot, Jilin Prov Key Lab Anim Microecol & Hlth Breeding, Key Lab Anim Prod & Prod Quality Safety,Minist Edu, Changchun, Peoples R China

[2] Natl Res Ctr, Vet Res Inst, Parasitol & Anim Dis Dept, Cairo 12622, Egypt

来源：

RESEARCH IN VETERINARY SCIENCE | 2022年 / 153卷

基金：

中国国家自然科学基金;

关键词：

Lactobacillus plantarum; Influenza virus; FLIC flagellin; Mucosal immunity; PROTECTIVE EFFICACY; IMMUNE-RESPONSES; VACCINES; NUCLEOPROTEIN; PROTEINS; NP;

D O I：

10.1016/j.rvsc.2022.10.024

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Avian influenza virus (AIV)is easy to cause diseases in birds and humans.It causes great economic losses to the poultry farms and leads to public health problems. Using vaccines is the main approach to control the prevalence of AIV. In our previously published article, a recombinant Lactobacillus plantarum (L. plantarum) expressing the NP-M2 peptide ofH9N2 AIV was generated, and its protective effect was evaluated in a chicken model. In this study, the protective effect was estimated in mice model. Humoral and cellular immune response parameters were measured using flow cytometry adding to body weight loss, survival rate, virus load, and histopathological changes in the lung. The obtained results elucidated that, the recombinant L. plantarum can promote the acti-vation of dendritic cells (DC), proliferation of T and B cells adding to eliciting protective secretory IgA (sIgA) and humeral IgG level in mice model. Accordingly, it could be used as a patent vaccine to control the AIV infection.

引用

页码：115 / 126

页数：12

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