Effect of yellow capsicum extract on proliferation and differentiation of 3T3-L1 preadipocytes

Objectives: To evaluate the effect of effect of Yellow Capsicum extract (YCE) that is rich in capsaicin on the proliferation and differentiation of 3T3-L1 preadipocytes in vitro. Methods: 3T3 L1 cells that were exposed to differentiation-inducing medium containing high glucose DMEM (Dulbecco's Modified Eagle's Medium) and subsequently were treated with capsaicin and YCE for their effect on adipocyte differentiation, changes in their triglyceride content, leptin secretion, expression of lipoprotein lipase, PPAR gamma, and CCAAT/enhancer-binding protein alpha (C/EBP alpha). Results: Both YCE and capsaicin inhibited proliferation and differentiation 3T3-L1 preadipocytes and suppressed accumulation of intracellular triglyceride in a dose-dependent manner. In addition, a significant decrease in the expression of lipoprotein lipase (LPL), leptin, PPAR gamma, and C/EBP alpha was noted in 3T3-L1 preadipocytes when induced to differentiate by YCE and Capsaicin. Conclusions: The potent inhibitory action of YCE and Capsaicin on the differentiation of 3T3-L1 preadipocyte observed suggests that they (YCE and Capsaicin) have the potential to inhibit obesity that needs to be explored in future studies. (C) 2014 Elsevier Inc. All rights reserved.