Molecular cloning, functional expression and purification of a glucan branching enzyme from Neisseria denitrificans

被引：8

作者：

Büttcher, V ^{[1
]}

Quanz, M ^{[1
]}

Willmitzer, L ^{[1
]}

机构：

[1] Inst Genbiol Forsch Berlin GmbH, D-14195 Berlin, Germany

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1999年 / 1432卷 / 02期

关键词：

glucan branching enzyme; glycogen; alpha-1,4-glucans; alpha-1,6-glucans; Neisseria; cloning;

D O I：

10.1016/S0167-4838(99)00101-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nucleotide sequence containing the complete structural information fora glucan branching enzyme was isolated from a Neisseria denitrificans genomic library. The gene was expressed in Escherichia coli and the active recombinant protein was purified. The deduced protein of 762 amino acids with a calculated molecular weight of 86313 shows similarity to the primary protein sequences of other known glucan branching enzymes; Amino acid sequencing of the isolated protein by Edman degradation confirmed the deduced start codon, of the structural gene of the glucan branching enzyme. The purified glucan branching enzyme has a stimulating effect on the Neisseria amylosucrase activity. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：406 / 412

页数：7

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