A Dual Molecular Biointerface Combining RGD and KRSR Sequences Improves Osteoblastic Functions by Synergizing Integrin and Cell-Membrane Proteoglycan Binding

被引:24
作者
Hoyos-Nogues, Mireia [1 ,2 ,3 ]
Falgueras-Batlle, Elena [1 ,2 ]
Ginebra, Maria-Pau [1 ,2 ]
Maria Manero, Jose [1 ,2 ]
Gil, Javier [1 ,3 ]
Mas-Moruno, Carlos [1 ,2 ]
机构
[1] UPC, Dept Mat Sci & Met Engn, Biomat Biomech & Tissue Engn Grp BBT, Barcelona 08019, Spain
[2] UPC, Barcelona Res Ctr Multiscale Sci & Engn, Barcelona 08019, Spain
[3] UIC, Sant Cugat Del Valles 08195, Spain
关键词
biointerface; titanium; functionalization; RGD; integrin; KRSR; proteoglycan; osteointegration; osteoblast; coating; MULTIFUNCTIONAL COATINGS; PEPTIDES; BONE; TITANIUM; SURFACE; ADHESION; BIOMATERIALS; FUNCTIONALIZATION; DIFFERENTIATION; PEPTIDOMIMETICS;
D O I
10.3390/ijms20061429
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Synergizing integrin and cell-membrane heparan sulfate proteoglycan signaling on biomaterials through peptidic sequences is known to have beneficial effects in the attachment and behavior of osteoblasts; however, controlling the exact amount and ratio of peptides tethered on a surface is challenging. Here, we present a dual molecular-based biointerface combining integrin (RGD) and heparin (KRSR)-binding peptides in a chemically controlled fashion. To this end, a tailor-made synthetic platform (PLATF) was designed and synthesized by solid-phase methodologies. The PLATF and the control linear peptides (RGD or KRSR) were covalently bound to titanium via silanization. Physicochemical characterization by means of contact angle, Raman spectroscopy and XPS proved the successful and stable grafting of the molecules. The biological potential of the biointerfaces was measured with osteoblastic (Saos-2) cells both at short and long incubation periods. Biomolecule grafting (either the PLATF, RGD or KRSR) statistically improved (p < 0.05) cell attachment, spreading, proliferation and mineralization, compared to control titanium. Moreover, the molecular PLATF biointerface synergistically enhanced mineralization (p < 0.05) of Saos-2 cells compared to RGD or KRSR alone. These results indicate that dual-function coatings may serve to improve the bioactivity of medical implants by mimicking synergistic receptor binding.
引用
收藏
页数:13
相关论文
共 45 条
  • [11] A review on the wettability of dental implant surfaces II: Biological and clinical aspects
    Gittens, Rolando A.
    Scheideler, Lutz
    Rupp, Frank
    Hyzy, Sharon L.
    Geis-Gerstorfer, Juergen
    Schwartz, Zvi
    Boyan, Barbara D.
    [J]. ACTA BIOMATERIALIA, 2014, 10 (07) : 2907 - 2918
  • [12] The future of biologic coatings for orthopaedic implants
    Goodman, Stuart B.
    Yao, Zhenyu
    Keeney, Michael
    Yang, Fan
    [J]. BIOMATERIALS, 2013, 34 (13) : 3174 - 3183
  • [13] Micropatterned surfaces modified with select peptides promote exclusive interactions with osteoblasts
    Hasenbein, ME
    Andersen, TT
    Bizios, R
    [J]. BIOMATERIALS, 2002, 23 (19) : 3937 - 3942
  • [14] Tuning Mesenchymal Stem Cell Response onto Titanium-Niobium-Hafnium Alloy by Recombinant Fibronectin Fragments
    Herranz-Diez, C.
    Mas-Moruno, C.
    Neubauer, S.
    Kessler, H.
    Gil, F. J.
    Pegueroles, M.
    Manero, J. M.
    Guillem-Marti, J.
    [J]. ACS APPLIED MATERIALS & INTERFACES, 2016, 8 (04) : 2517 - 2525
  • [15] RGD modified polymers: biomaterials for stimulated cell adhesion and beyond
    Hersel, U
    Dahmen, C
    Kessler, H
    [J]. BIOMATERIALS, 2003, 24 (24) : 4385 - 4415
  • [16] All-in-one trifunctional strategy: A cell adhesive, bacteriostatic and bactericidal coating for titanium implants
    Hoyos-Nogues, Mireia
    Buxadera-Palomero, Judit
    Ginebra, Maria-Pau
    Maria Manero, Jose
    Gil, Fj
    Mas-Moruno, Carlos
    [J]. COLLOIDS AND SURFACES B-BIOINTERFACES, 2018, 169 : 30 - 40
  • [17] Regenerating Bone via Multifunctional Coatings: The Blending of Cell Integration and Bacterial Inhibition Properties on the Surface of Biomaterials
    Hoyos-Nogues, Mireia
    Velasco, Ferran
    Ginebra, Maria-Pau
    Maria Manero, Jose
    Javier Gil, F.
    Mas-Moruno, Carlos
    [J]. ACS APPLIED MATERIALS & INTERFACES, 2017, 9 (26) : 21618 - 21630
  • [18] A Comprehensive Evaluation of the Activity and Selectivity Profile of Ligands for RGD-binding Integrins
    Kapp, Tobias G.
    Rechenmacher, Florian
    Neubauer, Stefanie
    Maltsev, Oleg V.
    Cavalcanti-Adam, Elisabetta A.
    Zarka, Revital
    Reuning, Ute
    Notni, Johannes
    Wester, Hans-Juergen
    Mas-Moruno, Carlos
    Spatz, Joachim
    Geiger, Benjamin
    Kessler, Horst
    [J]. SCIENTIFIC REPORTS, 2017, 7
  • [19] Beyond RGD; nanoclusters of syndecan- and integrin-binding ligands synergistically enhance cell/material interactions
    Karimi, Fatemeh
    Thombare, Varsha Jagannath
    Hutton, Craig A.
    O'Connor, Andrea J.
    Qiao, Greg G.
    Heath, Daniel E.
    [J]. BIOMATERIALS, 2018, 187 : 81 - 92
  • [20] Effect of RGDS and KRSR Peptides Immobilized on Silk Fibroin Nanofibrous Mats for Cell Adhesion and Proliferation
    Kim, Jong Wook
    Ki, Chang Seok
    Park, Young Hwan
    Kim, Hyun Jeong
    Um, In Chul
    [J]. MACROMOLECULAR RESEARCH, 2010, 18 (05) : 442 - 448