A simple protein-based surrogate neutralization assay for SARS-CoV-2

被引：160

作者：

Abe, Kento T. ^{[1
,2
]}

Li, Zhijie ^{[1
]}

Samson, Reuben ^{[1
,2
]}

Samavarchi-Tehrani, Payman ^{[2
]}

Valcourt, Emelissa J. ^{[3
]}

Wood, Heidi ^{[3
]}

Budylowski, Patrick ^{[4
,5
]}

Dupuis, Alan P. ^{[6
]}

Girardin, Roxie C. ^{[6
]}

Rathod, Bhavisha ^{[2
]}

Wang, Jenny H. ^{[2
]}

Barrios-Rodiles, Miriam ^{[2
]}

Colwill, Karen ^{[2
]}

McGeer, Allison J. ^{[2
,7
,8
,9
,10
]}

Mubareka, Samira ^{[10
,11
,12
,13
]}

Gommerman, Jennifer L. ^{[4
]}

Durocher, Yves ^{[14
]}

Ostrowski, Mario ^{[4
,15
,16
]}

McDonough, Kathleen A. ^{[6
,17
]}

Drebot, Michael A. ^{[3
,18
]}

Drews, Steven J. ^{[19
,20
]}

Rini, James M. ^{[1
,21
]}

Gingras, Anne-Claude ^{[1
,2
]}

机构：

[1] Univ Toronto, Dept Mol Genet, Toronto, ON, Canada

[2] Sinai Hlth Syst, Lunenfeld Tanenbaum Res Inst, Mt Sinai Hosp, Toronto, ON, Canada

[3] Publ Hlth Agcy Canada, Natl Microbiol Lab NML, Zoonot Dis & Special Pathogens, Winnipeg, MB, Canada

[4] Univ Toronto, Dept Immunol, Toronto, ON, Canada

[5] Univ Toronto, Inst Med Sci, Toronto, ON, Canada

[6] New York State Dept Hlth, Wadsworth Ctr, Albany, NY USA

[7] Univ Hlth Network, Dept Microbiol, Toronto, ON, Canada

[8] Sinai Hlth Syst, Toronto, ON, Canada

[9] Univ Toronto, Dalla Lana Sch Publ Hlth, Toronto, ON, Canada

[10] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada

[11] Sunnybrook Hlth Sci Ctr, Dept Lab Med & Mol Diagnost, Div Microbiol, Toronto, ON, Canada

[12] Sunnybrook Res Inst, Biol Sci, Toronto, ON, Canada

[13] Sunnybrook Hlth Sci Ctr, Div Infect Dis, Toronto, ON, Canada

[14] Natl Res Council Canada, Mammalian Cell Express Human Hlth Therapeut Res C, Montreal, PQ, Canada

[15] Univ Toronto, Dept Med, Toronto, ON, Canada

[16] St Michaels Hosp, Li Ka Shing Knowledge Inst, Toronto, ON, Canada

[17] SUNY Albany, Sch Publ Hlth, Dept Biomed Sci, Albany, NY USA

[18] Univ Manitoba, Dept Med Microbiol & Infect Dis, Winnipeg, MB, Canada

[19] Canadian Blood Serv, Edmonton, AB, Canada

[20] Univ Alberta, Dept Lab Med & Pathol, Edmonton, AB, Canada

[21] Univ Toronto, Dept Biochem, Toronto, ON, Canada

来源：

JCI INSIGHT | 2020年 / 5卷 / 19期

基金：

加拿大健康研究院; 加拿大创新基金会;

关键词：

CORONAVIRUS; PIGGYBAC;

D O I：

10.1172/jci.insight.142362

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Most of the patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mount a humoral immune response to the virus within a few weeks of infection, but the duration of this response and how it correlates with clinical outcomes has not been completely characterized. Of particular importance is the identification of immune correlates of infection that would support public health decision-making on treatment approaches, vaccination strategies, and convalescent plasma therapy. While ELISA-based assays to detect and quantitate antibodies to SARS-CoV-2 in patient samples have been developed, the detection of neutralizing antibodies typically requires more demanding cell-based viral assays. Here, we present a safe and efficient protein-based assay for the detection of serum and plasma antibodies that block the interaction of the SARS-CoV-2 spike protein receptor binding domain (RBD) with its receptor, angiotensin-converting enzyme 2 (ACE2). The assay serves as a surrogate neutralization assay and is performed on the same platform and in parallel with an ELISA for the detection of antibodies against the RBD, enabling a direct comparison. The results obtained with our assay correlate with those of 2 viral-based assays, a plaque reduction neutralization test (PRNT) that uses live SARS-CoV-2 virus and a spike pseudotyped viral vector-based assay.

引用

页数：14

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