Sofosbuvir shows a protective effect against vertical transmission of Zika virus and the associated congenital syndrome in rhesus monkeys

被引：18

作者：

Gardinali, Noemi R. ^{[1
]}

Marchevsky, Renato S. ^{[2
]}

Oliveira, Jaqueline M. ^{[1
]}

Pelajo-Machado, Marcelo ^{[3
]}

Kugelmeier, Tatiana ^{[4
]}

Castro, Marcio P. ^{[5
]}

Silva, Aline C. A. ^{[6
]}

Pinto, Douglas P. ^{[6
]}

Fonseca, Lais B. ^{[6
]}

Vilhena, Leandro S. ^{[6
]}

Pereira, Heliana M. ^{[6
]}

Lima, Sheila M. B. ^{[7
]}

Miranda, Emily H. ^{[7
]}

Trindade, Gisela F. ^{[7
]}

Linhares, Jose H. R. ^{[7
]}

Silva, Stephanie A. ^{[7
]}

Melgaco, Juliana Gil ^{[1
]}

Alves, Ada M. B. ^{[8
]}

Moran, Julio ^{[9
]}

Silva, Maria C. C. ^{[10
]}

Soares-Bezerra, Romulo J. ^{[1
]}

Soriano, Andreza ^{[1
]}

Bentes, Gentil A. ^{[1
]}

Bottino, Fernanda O. ^{[1
]}

Salvador Castro Faria, Sarah Beatriz ^{[1
]}

Nudelman, Rafael F. ^{[4
]}

Lopes, Claudia A. A. ^{[4
]}

Perea, Javier A. S. ^{[4
]}

Sarges, Klena ^{[4
]}

Andrade, Marcia C. R. ^{[4
]}

Motta, Marcia C. V. A. ^{[7
]}

Freire, Marcos S. ^{[7
]}

Souza, Thiago M. L. ^{[11
]}

Schmidt-Chanasit, Jonas ^{[12
]}

Pinto, Marcelo A. ^{[1
]}

机构：

[1] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Desenvolvimento Tecnol Virol, Ave Brasil 4365, Rio De Janeiro, RJ, Brazil

[2] Fundacao Oswaldo Cruz, Inst Tecnol Imunobiol Biomanguinhos, Lab Neurovirulencia, Ave Brasil 4365, Rio De Janeiro, RJ, Brazil

[3] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Patol, Ave Brasil 4365, BR-4365 Rio De Janeiro, RJ, Brazil

[4] Fundacao Oswaldo Cruz, Inst Ciencia & Tecnol Biomodelos, Ave Brasil 4365,Ave Brasil, Rio De Janeiro, RJ, Brazil

[5] Ctr Diagnost Vet CEVET, Ave Rui Barbosa 29, Niteroi, RJ, Brazil

[6] Fundacao Oswaldo Cruz, Serv Equivalencia & Farmacocinet SEFAR, Prod & Inovacao Saude, Ave Brasil 4365, Rio De Janeiro, RJ, Brazil

[7] Fundacao Oswaldo Cruz, Inst Tecnol Imunobiol Biomanguinhos, Lab Tecnol Virol, Ave Brasil 4365, BR-4365 Rio De Janeiro, RJ, Brazil

[8] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Biotecnol & Fisiol Infeccoes Virais, Ave Brasil 4365, BR-4365 Rio De Janeiro, RJ, Brazil

[9] Dr Julio Moran Labs, Vordergrut 30, Zurich, Switzerland

[10] Fed Univ ABC, Ctr Ciencias Nat & Humanas, Lab Biol Mol Patogenos, Ave Estados 5001, Sao Bernardo Do Campo, SP, Brazil

[11] Fiocruz MS, Inst Nacl Ciencia & Tecnol Gestao Inovacao Doenca, Ctr Desenvolvimento Tecnol Saude, Ave Brasil 4365, Rio De Janeiro, RJ, Brazil

[12] Bernhard Nocht Inst Trop Med, WHO Collaborating Ctr Arbovirus & Haemorrhag Feve, Bernhard Nocht Str 74, Hamburg, Germany

来源：

ANTIVIRAL RESEARCH | 2020年 / 182卷

关键词：

Sofosbuvir; Zika virus; Pregnancy; Rhesus monkeys; Congenital Zika virus syndrome; VIRAL-INFECTION; PREGNANCY; INFLAMMATION; CELLS; ANTIBODIES; DIAGNOSIS; FETUSES; MODEL;

D O I：

10.1016/j.antiviral.2020.104859

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The outbreaks of Zika virus (ZIKV) infection in Brazil, 2015-2016, were associated with severe congenital malformations. Our translational study aimed to test the efficacy of the antiviral agent sofosbuvir (SOF) against vertical transmission of ZIKV and the associated congenital syndrome (CZS), using a rhesus monkey model. Eight pregnant macaques were successfully infected during the organogenesis phase with a Brazilian ZIKV strain; five of them received SOF from two to fifteen days post-infection. Both groups of dams showed ZIKV-associated clinical signals, detectable ZIKV RNA in several specimens, specific anti-ZIKV IgM and IgG antibodies, and maternal neutralizing antibodies. However, malformations occurred only among non-treated dam offspring. Compared to non-treated animals, all SOF-treated dams had a shorter ZIKV viremia and four of five neonates had undetectable ZIKV RNA in blood and tissue samples. These results support further clinical evaluations aiming for the prevention of CZS.

引用

页数：19

共 78 条

[1]

ABITBOL MM, 1977, AM J PATHOL, V86, P573

[2]

Agudelo-Salas Ivony Y., 2017, Rev. salud pública, V19, P460, DOI 10.15446/rsap.v19n4.39597

[3]

Amorim P, 2016, ERCIM NEWS, P10

[4]

[Anonymous], 2018, NAT COMMUN, DOI DOI 10.1038/S41467-018-9

[5]

[Anonymous], J INNATE IMMUN

[6]

[Anonymous], 2016, SCI REP, DOI DOI 10.1038/SREP39775

[7] Congenital Zika syndrome is associated with maternal protein malnutrition [J].

Barbeito-Andres, J. ;

Pezzuto, P. ;

Higa, L. M. ;

Dias, A. A. ;

Vasconcelos, J. M. ;

Santos, T. M. P. ;

Ferreira, J. C. C. G. ;

Ferreira, R. O. ;

Dutra, F. F. ;

Rossi, A. D. ;

Barbosa, R., V ;

Amorim, C. K. N. ;

De Souza, M. P. C. ;

Chimelli, L. ;

Aguiar, R. S. ;

Gonzalez, P. N. ;

Lara, F. A. ;

Castro, M. C. ;

Molnar, Z. ;

Lopes, R. T. ;

Bozza, M. T. ;

Vianez, J. L. S. G. ;

Barbeito, C. G. ;

Cuervo, P. ;

Bellio, M. ;

Tanuri, A. ;

Garcez, P. P. .

SCIENCE ADVANCES, 2020, 6 (02)

[8]

Barbeito-Andres J., 2018, 43 ANN M HUM BIOL AS

[9] Brain Structural Networks in Mouse Exposed to Chronic Maternal Undernutrition [J].

Barbeito-Andres, Jimena ;

Gleiser, Pablo M. ;

Bernal, Valeria ;

Hallgrimsson, Benedikt ;

Gonzalez, Paula N. .

NEUROSCIENCE, 2018, 380 :14-26

[10] Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus [J].

Barreto-Vieira, Debora Ferreira ;

Jacome, Fernanda Cunha ;

Nunes da Silva, Marcos Alexandre ;

Caldas, Gabriela Cardoso ;

Bispo de Filippis, Ana Maria ;

de Sequeira, Patricia Carvalho ;

de Souza, Elen Mello ;

Andrade, Audrien Alves ;

de Abreu Manso, Pedro Paulo ;

Trindade, Gisela Freitas ;

Barbosa Lima, Sheila Maria ;

Barth, Ortrud Monika .

PLOS ONE, 2017, 12 (09)

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