Mouse parenchymal liver cells in culture secrete a growth inhibitor for myeloma cells

被引：6

作者：

Takahashi, M

Yokomuro, K

机构：

[1] Nippon Medical School, Dept. of Microbiology and Immunology, Tokyo

[2] Nippon Medical School, Dept. of Microbiology and Immunology, Tokyo 113, 1-1-5, Sedagi, Bunkyo-ku

来源：

JOURNAL OF HEPATOLOGY | 1996年 / 24卷 / 02期

关键词：

cytokines; growth inhibitor; myeloma cells; parenchymal liver cells;

D O I：

10.1016/S0168-8278(96)80033-X

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Methods: Growth inhibitory activity in the conditioned medium of mouse parenchymal liver cells was examined in three strains of myeloma cells. Results: Two strains of myeloma cells were highly sensitive to a low concentration of mouse parenchymal liver cell derived growth inhibitor, whereas one strain was resistant to the same concentration. Interferon-alpha beta and transforming growth factor-beta activity were detected in mouse parenchymal liver cells, while interferon-gamma and tumor necrosis factor-alpha were not. The growth suppression exerted by mouse parenchymal liver cell derived growth inhibitor in the three myeloma strains was distinct from that exerted by transforming growth factor-beta, tumor necrosis factor-alpha, interferon-alpha beta and interferon-gamma. The mouse parenchymal liver cell derived growth inhibitor was eluted with a peak activity in the 18 kDa range and focused into pI values of 3.8-4.0, and it was lost when mouse parenchymal liver cells were treated with heat or trypsin. Conclusion: These results indicate that mouse parenchymal liver cell derived growth inhibitor differs from the well-characterized growth inhibitors, transforming growth factor-beta, tumor necrosis factor-alpha, interferon-alpha beta and interferon-gamma.

引用

页码：225 / 229

页数：5

共 19 条

[1] THE LIVER AND IGA - IMMUNOLOGICAL, CELL BIOLOGICAL AND CLINICAL IMPLICATIONS [J].