An Image Analysis Method for the Precise Selection and Quantitation of Fluorescently Labeled Cellular Constituents: Application to the Measurement of Human Muscle Cells in Culture

被引：54

作者：

Agley, Chibeza C. ^{[1
]}

Velloso, Cristiana P. ^{[1
]}

Lazarus, Norman R. ^{[1
]}

Harridge, Stephen D. R. ^{[1
]}

机构：

[1] Kings Coll London, Ctr Human & Aerosp Physiol Sci, Sch Biomed Sci, London SE1 1UL, England

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2012年 / 60卷 / 06期

关键词：

human muscle; myosin heavy chain; myotubes; myotube size; myotube area; myotube diameter; semiquantitative image analysis; layers; fluorescence intensity; MYOTUBE FORMATION; MYOGENIC DIFFERENTIATION; MYOBLAST FUSION; GROWTH-FACTORS; SKELETAL; EXPRESSION; MYOSTATIN; SIZE; TRANSPLANTATION; PROLIFERATION;

D O I：

10.1369/0022155412442897

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The accurate measurement of the morphological characteristics of cells with nonuniform conformations presents difficulties. We report here a straightforward method using immunofluorescent staining and the commercially available imaging program Adobe Photoshop, which allows objective and precise information to be gathered on irregularly shaped cells. We have applied this measurement technique to the analysis of human muscle cells and their immunologically marked intracellular constituents, as these cells are prone to adopting a highly branched phenotype in culture. Use of this method can be used to overcome many of the long-standing limitations of conventional approaches for quantifying muscle cell size in vitro. In addition, wider applications of Photoshop as a quantitative and semiquantitative tool in immunocytochemistry are explored. (J Histochem Cytochem 60:428-438, 2012)

引用

页码：428 / 438

页数：11

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