NMR assignment of human granulocyte-macrophage colony-stimulating factor

被引：8

作者：

Sauve, Simon ^{[1
]}

Gingras, Genevieve ^{[1
]}

Aubin, Yves ^{[1
,2
,3
,4
]}

机构：

[1] Hlth Canada, Biol Res Ctr, Biol & Genet Therapies Directorate, Ottawa, ON K1A 0K9, Canada

[2] Carleton Univ, Dept Chem, Ottawa, ON K1S 5B6, Canada

[3] Univ Ottawa, Dept Biochem Mol Biol & Immunol, Ottawa, ON K1H 8M5, Canada

[4] Univ Montreal, Dept Biochem, Montreal, PQ H3T 1J4, Canada

来源：

BIOMOLECULAR NMR ASSIGNMENTS | 2008年 / 2卷 / 01期

关键词：

granulocyte-macrophage colony-stimulating factor; recombinant protein therapeutics; protein characterization;

D O I：

10.1007/s12104-007-9070-8

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Human granulocyte-macrophage colony-stimulating factor (hGM-CSF), also known as sargramostim or molgramostin, is a cytokine that functions as a hematopoietic cell growth factor. Here we report a near complete assignment for the backbone and side chain resonances for the mature polypeptide.

引用

页码：5 / 7

页数：3

共 8 条

[1] Protein backbone angle restraints from searching a database for chemical shift and sequence homology [J].

Cornilescu, G ;

Delaglio, F ;

Bax, A .

JOURNAL OF BIOMOLECULAR NMR, 1999, 13 (03) :289-302

[2] NMRPIPE - A MULTIDIMENSIONAL SPECTRAL PROCESSING SYSTEM BASED ON UNIX PIPES [J].

DELAGLIO, F ;

GRZESIEK, S ;

VUISTER, GW ;

ZHU, G ;

PFEIFER, J ;

BAX, A .

JOURNAL OF BIOMOLECULAR NMR, 1995, 6 (03) :277-293

[3] NMR VIEW - A COMPUTER-PROGRAM FOR THE VISUALIZATION AND ANALYSIS OF NMR DATA [J].

JOHNSON, BA ;

BLEVINS, RA .

JOURNAL OF BIOMOLECULAR NMR, 1994, 4 (05) :603-614

[4]

Rozwarski DA, 1996, PROTEINS, V26, P304, DOI 10.1002/(SICI)1097-0134(199611)26:3<304::AID-PROT6>3.0.CO

[5]

2-D

[6] Smartnotebook: A semi-automated approach to protein sequential NMR resonance assignments [J].

Slupsky, CM ;

Boyko, RF ;

Booth, VK ;

Sykes, BD .

JOURNAL OF BIOMOLECULAR NMR, 2003, 27 (04) :313-321

[7] 3-DIMENSIONAL STRUCTURE OF RECOMBINANT HUMAN GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR [J].

WALTER, MR ;

COOK, WJ ;

EALICK, SE ;

NAGABHUSHAN, TL ;

TROTTA, PP ;

BUGG, CE .

JOURNAL OF MOLECULAR BIOLOGY, 1992, 224 (04) :1075-1085

[8] Human prion proteins expressed in Escherichia coli and purified by high-affinity column refolding [J].

Zahn, R ;

vonSchroetter, C ;

Wuthrich, K .

FEBS LETTERS, 1997, 417 (03) :400-404

← 1 →