Two-step enzymatic functionalisation of polyamide with phenolics

被引：30

作者：

Acero, Enrique Herrero ^{[1
]}

Ribitsch, Doris ^{[1
]}

Rodriguez, Rosario Diaz ^{[2
]}

Dellacher, Anita ^{[5
]}

Zitzenbacher, Sabine ^{[1
]}

Marold, Annemarie ^{[1
]}

Greimel, Katrin J. ^{[1
]}

Schroeder, Marc ^{[2
]}

Kandelbauer, Andreas ^{[3
,4
]}

Heumann, Sonja ^{[2
]}

Nyanhongo, Gibson S. ^{[2
]}

Schwab, Helmut ^{[1
,5
]}

Guebitz, Georg M. ^{[1
,2
]}

机构：

[1] ACIB GmbH, A-8010 Graz, Austria

[2] Graz Univ Technol, Inst Environm Biotechnol, A-8010 Graz, Austria

[3] Kompetenzzentrum Holz GmbH, Wood Carinthian Competence Ctr W3C, A-9300 St Veit An Der Glan, Austria

[4] Univ Nat Resources & Appl Life Sci, Dept Wood Sci & Technol, A-1197 Vienna, Austria

[5] Graz Univ Technol, Inst Mol Biotechnol, A-8010 Graz, Austria

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2012年 / 79卷

关键词：

Polyamide; Polyamidase; Laccase; Enzymatic grafting; SURFACE HYDROLYSIS; ARYL ACYLAMIDASE; POLYETHYLENE TEREPHTHALATE; LACCASE; PURIFICATION; MEMBRANES; FIBERS; POLY(ETHYLENE-TEREPHTHALATE); POLYETHYLENETEREPHTHALATE; DEGRADATION;

D O I：

10.1016/j.molcatb.2012.03.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A two step enzymatic process for grafting phenolics onto polyamides (PAs) was developed in order to impart special functionalities to inert PA. Therefore, a polyamidase (NfpolyA) from Nocardia farcinica was overexpressed in Escherichia coli BL21-Gold(DE3) and purified in a single step. With p-nitroacetanilide as a substrate, NfpolyA revealed a specific activity of 20 U mg(-1) compared to 1.5 U mg(-1) for the wild-type enzyme. NfpolyA showed a K-M value of 0.12 +/- 0.01 mM and a k(cat) of 19.1 s(-1) which were both higher than measured for the wild-type enzyme (k(cat) = 3.5 s(-1); K-M = 0.06 mM). A laccase from Trametes hirsuta was used to oxidize ferulic acid, used as a phenol model substrate and study the covalent grafting of n-butylamine, as model substrate for PA. According to LC-MS, up to three equivalents of n-butylamine were bound to ferulic acid after laccase oxidation of ferulic acid. Both enzymes were used sequentially in a two step process. In a first step the polyamidase is used to partially hydrolyze the amide bond, leading to a surface with amine and carboxylic acids. In a second step, by using a laccase from T. hirsuta ferulic acid was grafted onto the surface of PA as confirmed with FTIR-ATR analysis. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：54 / 60

页数：7

共 61 条

[1] Enzymatic Surface Hydrolysis of PET: Effect of Structural Diversity on Kinetic Properties of Cutinases from Thermobifida [J].