Flotillin-1 stabilizes caveolin-1 in intestinal epithelial cells

被引:43
作者
Vassilieva, Elena V. [1 ]
Ivanov, Andrei I. [2 ]
Nusrat, Asma [1 ]
机构
[1] Emory Univ, Dept Pathol & Lab Med, Epithelial Pathobiol Res Unit, Atlanta, GA 30322 USA
[2] Univ Rochester, Dept Med, Div Gastroenterol & Hepatol, Rochester, NY 14642 USA
关键词
Epithelial cells; Lipid rafts; Lactosyl ceramide internalization; Lysosomal degradation; LIPID RAFTS; REGGIE/FLOTILLIN PROTEINS; MEMBRANE MICRODOMAINS; ENDOCYTIC PATHWAY; INTERNALIZATION; PROTEASOME; EXPRESSION; INHIBITORS; DYNAMICS; MICE;
D O I
10.1016/j.bbrc.2008.12.118
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Flotillins and caveolins represent two types of resident proteins associated with lipid rafts in mammalian cells, however, their possible cross-talk in regulating lipid raft functions remains poorly understood. In this report, we observed that siRNA-mediated down-regulation of flotillin-1 expression which disrupted lipid raft-mediated endocytosis of BODIPY FL C-5-lactosylceramide also substantially decreased caveolin-1 level in SK-CO 15 human intestinal epithelial cells. The decrease in caveolin-1 expression appeared to be specific for flotillin-1 knock-down and was not observed after down-regulation of flotillin-2. The decrease in caveolin-1 level in flotillin-1-depleted cells was not due to suppression of its mRNA synthesis and was not mimicked by cholesterol depletion of SK-CO15 cells. Furthermore, flotillin-1 dependent down-regulation of caveolin-1 was reversed after cell exposure to lysosomal inhibitor, chloroquine but not proteosomal inhibitor, MG262. Our data suggest that flotillin-1 regulates caveolin-1 level by preventing its lysosomal degradation in intestinal epithelial cells. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码:460 / 465
页数:6
相关论文
共 39 条
[1]   Potent and selective inhibitors of the proteasome: Dipeptidyl boronic acids [J].
Adams, J ;
Behnke, M ;
Chen, SW ;
Cruickshank, AA ;
Dick, LR ;
Grenier, L ;
Klunder, JM ;
Ma, YT ;
Plamondon, L ;
Stein, RL .
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1998, 8 (04) :333-338
[2]   Chemokines in autoinimune lacrimal gland disease in MRL/MpJ mice [J].
Akpek, EK ;
Jabs, DA ;
Gérard, HC ;
Prendergast, RA ;
Hudson, AP ;
Lee, B ;
Whittum-Hudson, JA .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2004, 45 (01) :185-190
[3]  
BABUKE T, 2007, EUR J CELL BIOL
[4]   A new paradigm for membrane-organizing and -shaping scaffolds [J].
Bauer, Manuel ;
Pelkmans, Lucas .
FEBS LETTERS, 2006, 580 (23) :5559-5564
[5]  
BLANCHER MH, 2008, CRIPTO RECRUITS FURI
[6]   The SPFH domain-containing proteins: more than lipid raft markers [J].
Browman, Duncan T. ;
Hoegg, Maja B. ;
Robbins, Stephen M. .
TRENDS IN CELL BIOLOGY, 2007, 17 (08) :394-402
[7]   Knockdown of flotillin-2 inhibits lung surfactant secretion by alveolar type II cells [J].
Chintagari, Narendranath Reddy ;
Gou, Deming ;
Liu, Lin .
CELL RESEARCH, 2008, 18 (06) :701-703
[8]   Loss of caveolae, vascular dysfunction, and pulmonary defects in caveolin-1 gene-disrupted mice [J].
Drab, M ;
Verkade, P ;
Elger, M ;
Kasper, M ;
Lohn, M ;
Lauterbach, B ;
Menne, J ;
Lindschau, C ;
Mende, F ;
Luft, FC ;
Schedl, A ;
Haller, H ;
Kurzchalia, TV .
SCIENCE, 2001, 293 (5539) :2449-2452
[9]  
Evans WE, 2003, J ANDROL, V24, P812
[10]   Spatial and temporal regulation of GLUT4 translocation by flotillin-1 and caveolin-3 in skeletal muscle cells [J].
Fecchi, Katia ;
Volonte, Daniela ;
Hezel, Michael P. ;
Schmeck, Kevin ;
Galbiati, Ferruccio .
FASEB JOURNAL, 2006, 20 (02) :705-+