Thermal characteristics and cadmium binding behavior of EC-ELP fusion polypeptides

被引：8
作者：
Choi, Heelak [1 ]
Han, Sung-Jin [1 ]
Won, Jong-In [1 ]
机构：
[1] Hongik Univ, Dept Chem Engn, Seoul 04066, South Korea
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 2020年 / 140卷
基金：
新加坡国家研究基金会;
关键词：
Elastin-like polypeptide (ELP); Synthetic phytochelatin (EC); Cadmium adsorption; EC-ELP; HEAVY-METALS; METALLOTHIONEINS; PEPTIDES; PROTEINS; DOMAIN;
D O I：
10.1016/j.enzmictec.2020.109628
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Elastin-like polypeptides (ELPs) are stimulus-responsive protein-based biopolymers that exhibit phase transition behavior. By joining them to synthetic phytochelatin (EC), EC-ELP fusion proteins with temperature sensitivity and metal-binding functionality were generated to remove heavy metal ions biologically. Three different EC domains (EC10, EC20, EC30) were incorporated into the ELP, and the EC-ELP fusion proteins were expressed in E. coll. Their thermal properties and metal binding abilities were then investigated according to the EC length. In addition, the feasibility of reusing EC-ELPs and the cadmium ion binding affinity of reused EC-ELPs were explored.
引用
收藏
页数：5
相关论文
共 29 条
  • [1] Characterization of the ars Gene Cluster from Extremely Arsenic-Resistant Microbacterium sp Strain A33
    Achour-Rokbani, Asma
    Cordi, Audrey
    Poupin, Pascal
    Bauda, Pascale
    Billard, Patrick
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2010, 76 (03) : 948 - 955
  • [2] Recent findings on the phytoremediation of soils contaminated with environmentally toxic heavy metals and metalloids such as zinc, cadmium, lead, and arsenic
    Alkorta I.
    Hernández-Allica J.
    Becerril J.M.
    Amezaga I.
    Albizu I.
    Garbisu C.
    [J]. Reviews in Environmental Science and Biotechnology, 2004, 3 (1) : 71 - 90
  • [3] Metal-binding characteristics of a phytochelatin analog (Glu-Cys)(2)Gly
    Bae, W
    Mehra, RK
    [J]. JOURNAL OF INORGANIC BIOCHEMISTRY, 1997, 68 (03) : 201 - 210
  • [4] Bae W, 2000, BIOTECHNOL BIOENG, V70, P518, DOI 10.1002/1097-0290(20001205)70:5<518::AID-BIT6>3.0.CO
  • [5] 2-5
  • [6] Synthesis and characterization of an ELP-conjugated liposome with thermo-sensitivity for controlled release of a drug
    Choi, Heelak
    Chu, Hun-Su
    Chung, Minsub
    Kim, Bumsang
    Won, Jong-In
    [J]. BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, 2016, 21 (05) : 620 - 626
  • [7] Cadmium detection by a thermally responsive elastin copolymer with metal-binding functionality
    Chu, Hun Su
    Ryum, Jung
    Won, Jong-In
    [J]. ENZYME AND MICROBIAL TECHNOLOGY, 2013, 53 (03) : 189 - 193
  • [8] CHARACTERIZATION OF PEPTIDE-COATED CADMIUM-SULFIDE CRYSTALLITES
    DAMERON, CT
    WINGE, DR
    [J]. INORGANIC CHEMISTRY, 1990, 29 (07) : 1343 - 1348
  • [9] GEKELER W, 1989, Z NATURFORSCH C, V44, P361
  • [10] Phytochelatin synthase genes from arabidopsis and the yeast Schizosaccharomyces pombe
    Ha, SB
    Smith, AP
    Howden, R
    Dietrich, WM
    Bugg, S
    O'Connell, MJ
    Goldsbrough, PB
    Cobbett, CS
    [J]. PLANT CELL, 1999, 11 (06) : 1153 - 1163
123