Half-lives of ornithine decarboxylase and S-adenosylmethionine decarboxylase activities during the cell cycle of Chinese hamster ovary cells

Cells in mitosis were seeded immediately after being harvested by the mitotic shake off technique from a culture of exponentially growing Chinese hamster ovary cells. At 2, 5, 7, 10, and 12 h after seeding, cycloheximide was added. Cells were sampled at various times after cycloheximide addition and the ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC) activities were determined. Flow cytometric analysis showed that cells sampled at 2, 5, 7, 10, and 12 h after seeding were found in mid G(1), at the G(1)/S transition, in mid S phase, at the S/G(2) transition, and in late G(2), respectively. The half-lives of ODC and AdoMetDC activities varied during the cell cycle. The half-life of ODC activity showed a biphasic pattern with increases in connection to the G(1)/S and S/G(2) transitions while the half-life of AdoMetDC activity increased only at the G(1)/S transition. (C) 1999 Academic Press.