A new approach to the role of IL-7 and TGF-β in the in vitro generation of thymus-derived CD4+CD25+Foxp3+regulatory T cells

被引：3
|
作者：
Bienkowska, Anna [1 ]
Kiernozek, Ewelina [1 ]
Kozlowska, Ewa [1 ]
Bugajski, Lukasz [2 ]
Drela, Nadzieja [1 ]
机构：
[1] Univ Warsaw, Fac Biol, Dept Immunol, Miecznikowa 1, PL-02096 Warsaw, Poland
[2] Nencki Inst Expt Biol PAS, Lab Cytometry, 3 Pasteur St, PL-02093 Warsaw, Poland
来源：
CYTOKINE | 2018年 / 102卷
关键词：
Cytokines; Dendritic cells; Regulatory T cells; Thymus-derived tregs; IMMUNOLOGICAL SELF-TOLERANCE; AUTOIMMUNE-DISEASES; REGULATORY CELLS; MAINTENANCE; PROLIFERATION; HOMEOSTASIS; MECHANISMS; SURVIVAL; SIGNALS; NAIVE;
D O I：
10.1016/j.cyto.2017.07.019
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Thymus-derived regulatory T cells of CD4 + CD25 + Foxp3 + phenotype develop as a functional, mature population playing an essential role in self-tolerance and immune homeostasis, and exhibiting therapeutic potential to inhibit adverse immune response. Despite intensive research on thymus-derived Tregs, the knowledge about agents involved in their generation, survival, proliferation, and biological functions is still insufficient. In this research we have focused on the role of selected cytokines in previously developed in vitro model based on the application of anti-CD3 monoclonal antibodies. We have demonstrated an essential role of IL-7 and TGF-beta in the generation of thymus-derived Tregs in the co-culture of thymocytes and JAWS II cells. In addition, in vitro generated Tregs exhibited their suppressive function similarly to Tregs sorted from freshly isolated thymus.
引用
收藏
页码：107 / 116
页数：10
相关论文
共 50 条
  • [41] Human Dendritic Cells Produce TGF-β1 under the Influence of Lung Carcinoma Cells and Prime the Differentiation of CD4+CD25+Foxp3+ Regulatory T Cells
    Dumitriu, Ingrid E.
    Dunbar, Donald R.
    Howie, Sarah E.
    Sethi, Tariq
    Gregory, Christopher D.
    JOURNAL OF IMMUNOLOGY, 2009, 182 (05): : 2795 - 2807
  • [42] Immune Tolerance of Human Dental Pulp-Derived Mesenchymal Stem Cells Mediated by CD4+CD25+ FoxP3+ Regulatory T-Cells and Induced by TGF-β1 and IL-10
    Hong, Jong Won
    Lim, Jung Hyun
    Chung, Chooryung J.
    Kang, Tae Jo
    Kim, Tae Yeon
    Kim, Young Seok
    Roh, Tae Suk
    Lew, Dae Hyun
    YONSEI MEDICAL JOURNAL, 2017, 58 (05) : 1031 - 1039
  • [43] Induction of antigen specific CD4+CD25+Foxp3+T regulatory cells from naive natural thymic derived T regulatory cells
    Hall, Bruce M.
    Tran, Giang T.
    Robinson, Catherine M.
    Hodgkinson, Suzanne J.
    INTERNATIONAL IMMUNOPHARMACOLOGY, 2015, 28 (02) : 875 - 886
  • [44] Critical role of IL-2 and TGF-β in generation, function and stabilization of Foxp3+CD4+ Treg
    Horwitz, David A.
    Zheng, Song Guo
    Wang, Juhua
    Gray, J. Dixon
    EUROPEAN JOURNAL OF IMMUNOLOGY, 2008, 38 (04) : 912 - 915
  • [45] Are CD4+CD25-Foxp3+ cells in untreated new-onset lupus patients regulatory T cells?
    Yang, Hua-xia
    Zhang, Wen
    Zhao, Li-dan
    Li, Yang
    Zhang, Feng-chun
    Tang, Fu-lin
    He, Wei
    Zhang, Xuan
    ARTHRITIS RESEARCH & THERAPY, 2009, 11 (05)
  • [46] Increased CD4+CD25+FOXP3+ regulatory T cells in cancer patients from conversion of CD4+CD25- T cells through tumor-derived factors
    Mao, Chaoming
    Wang, Shengjun
    Jiang, Qian
    Tong, Jia
    Ma, Jie
    Yang, Min
    Xu, Xiaopeng
    Qiu, Gufeng
    Shao, Qixiang
    Li, Long
    Xu, Huaxi
    ONKOLOGIE, 2008, 31 (05): : 243 - 248
  • [47] Rapamycin combined with allogenic immature dendritic cells selectively expands CD4+CD25+Foxp3+ regulatory T cells in rats
    Wang, Guo-Ying
    Zhang, Qi
    Yang, Yang
    Chen, Wen-Jie
    Liu, Wei
    Jiang, Nan
    Chen, Gui-Hua
    HEPATOBILIARY & PANCREATIC DISEASES INTERNATIONAL, 2012, 11 (02) : 203 - 208
  • [48] Estrogen enhances the functions of CD4+CD25+Foxp3+ regulatory T cells that suppress osteoclast differentiation and bone resorption in vitro
    Luo, C. Y.
    Wang, L.
    Sun, C.
    Li, D. J.
    CELLULAR & MOLECULAR IMMUNOLOGY, 2011, 8 (01) : 50 - 58
  • [49] Altered Frequencies of CD4+ CD25+ Foxp3+ and CD8+ CD25+ Foxp3+ Regulatory T Cells in Pre-eclampsia
    Zare, Maryam
    Doroudchi, Mehrnoosh
    Gharesi-Fard, Behrouz
    IRANIAN JOURNAL OF ALLERGY ASTHMA AND IMMUNOLOGY, 2018, 17 (06) : 540 - 547
  • [50] Tumor-derived CD4+ CD25+ regulatory T cell suppression of dendritic cell function involves TGF-β and IL-10
    Larmonier, Nicolas
    Marron, Marilyn
    Zeng, Yi
    Cantrell, Jessica
    Romanoski, Angela
    Sepassi, Marjan
    Thompson, Sylvia
    Chen, Xinchun
    Andreansky, Samita
    Katsanis, Emmanuel
    CANCER IMMUNOLOGY IMMUNOTHERAPY, 2007, 56 (01) : 48 - 59
12345