A new approach to the role of IL-7 and TGF-β in the in vitro generation of thymus-derived CD4+CD25+Foxp3+regulatory T cells

被引：3
|
作者：
Bienkowska, Anna [1 ]
Kiernozek, Ewelina [1 ]
Kozlowska, Ewa [1 ]
Bugajski, Lukasz [2 ]
Drela, Nadzieja [1 ]
机构：
[1] Univ Warsaw, Fac Biol, Dept Immunol, Miecznikowa 1, PL-02096 Warsaw, Poland
[2] Nencki Inst Expt Biol PAS, Lab Cytometry, 3 Pasteur St, PL-02093 Warsaw, Poland
来源：
CYTOKINE | 2018年 / 102卷
关键词：
Cytokines; Dendritic cells; Regulatory T cells; Thymus-derived tregs; IMMUNOLOGICAL SELF-TOLERANCE; AUTOIMMUNE-DISEASES; REGULATORY CELLS; MAINTENANCE; PROLIFERATION; HOMEOSTASIS; MECHANISMS; SURVIVAL; SIGNALS; NAIVE;
D O I：
10.1016/j.cyto.2017.07.019
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Thymus-derived regulatory T cells of CD4 + CD25 + Foxp3 + phenotype develop as a functional, mature population playing an essential role in self-tolerance and immune homeostasis, and exhibiting therapeutic potential to inhibit adverse immune response. Despite intensive research on thymus-derived Tregs, the knowledge about agents involved in their generation, survival, proliferation, and biological functions is still insufficient. In this research we have focused on the role of selected cytokines in previously developed in vitro model based on the application of anti-CD3 monoclonal antibodies. We have demonstrated an essential role of IL-7 and TGF-beta in the generation of thymus-derived Tregs in the co-culture of thymocytes and JAWS II cells. In addition, in vitro generated Tregs exhibited their suppressive function similarly to Tregs sorted from freshly isolated thymus.
引用
收藏
页码：107 / 116
页数：10
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