Nonredundant Regulation of Rice Arbuscular Mycorrhizal Symbiosis by Two Members of the PHOSPHATE TRANSPORTER1 Gene Family

被引:262
|
作者
Yang, Shu-Yi [1 ]
Gronlund, Mette [2 ]
Jakobsen, Iver [2 ]
Grotemeyer, Marianne Suter [3 ]
Rentsch, Doris [3 ]
Miyao, Akio [4 ]
Hirochika, Hirohiko [4 ]
Kumar, Chellian Santhosh [5 ]
Sundaresan, Venkatesan [5 ]
Salamin, Nicolas [6 ]
Catausan, Sheryl [7 ]
Mattes, Nicolas [7 ]
Heuer, Sigrid [7 ]
Paszkowski, Uta [1 ]
机构
[1] Univ Lausanne, Dept Plant Mol Biol, CH-1015 Lausanne, Switzerland
[2] Tech Univ Denmark, Dept Chem & Biochem Engn, DK-4000 Roskilde, Denmark
[3] Univ Bern, Inst Plant Sci, CH-3013 Bern, Switzerland
[4] Natl Inst Agrobiol Sci, Agron Res Ctr, Tsukuba, Ibaraki 3058602, Japan
[5] Univ Calif Davis, Dept Plant Biol & Plant Sci, Davis, CA 95616 USA
[6] Univ Lausanne, Dept Ecol & Evolut, CH-1015 Lausanne, Switzerland
[7] Int Rice Res Inst, Plant Breeding Genet & Biotechnol Div, Manila 1301, Philippines
来源
PLANT CELL | 2012年 / 24卷 / 10期
基金
瑞士国家科学基金会; 美国国家科学基金会;
关键词
PHOSPHATE TRANSPORTER; DIFFERENTIAL REGULATION; PHOSPHORUS; EXPRESSION; PLANTS; FUNGI; ARABIDOPSIS; ROOTS; RESPONSES; PATHWAY;
D O I
10.1105/tpc.112.104901
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Pi acquisition of crops via arbuscular mycorrhizal (AM) symbiosis is becoming increasingly important due to limited high-grade rock Pi reserves and a demand for environmentally sustainable agriculture. Here, we show that 70% of the overall Pi acquired by rice (Oryza sativa) is delivered via the symbiotic route. To better understand this pathway, we combined genetic, molecular, and physiological approaches to determine the specific functions of two symbiosis-specific members of the PHOSPHATE TRANSPORTER1 (PHT1) gene family from rice, ORYsa;PHT1;11 (PT11) and ORYsa;PHT1;13 (PT13). The PT11 lineage of proteins from mono- and dicotyledons is most closely related to homologs from the ancient moss, indicating an early evolutionary origin. By contrast, PT13 arose in the Poaceae, suggesting that grasses acquired a particular strategy for the acquisition of symbiotic Pi. Surprisingly, mutations in either PT11 or PT13 affected the development of the symbiosis, demonstrating that both genes are important for AM symbiosis. For symbiotic Pi uptake, however, only PT11 is necessary and sufficient. Consequently, our results demonstrate that mycorrhizal rice depends on the AM symbiosis to satisfy its Pi demands, which is mediated by a single functional Pi transporter, PT11.
引用
收藏
页码:4236 / 4251
页数:16
相关论文
共 50 条
  • [1] Evolutionary conservation of a phosphate transporter in the arbuscular mycorrhizal symbiosis
    Karandashov, V
    Nagy, R
    Wegmüller, S
    Amrhein, N
    Bucher, M
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (16) : 6285 - 6290
  • [2] A Medicago truncatula phosphate transporter indispensable for the arbuscular mycorrhizal symbiosis
    Javot, Helene
    Penmetsa, R. Varma
    Terzaghi, Nadia
    Cook, Douglas R.
    Harrison, Maria J.
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (05) : 1720 - 1725
  • [3] Rice phosphate transporters include an evolutionarily divergent gene specifically activated in arbuscular mycorrhizal symbiosis
    Paszkowski, U
    Kroken, S
    Roux, C
    Briggs, SP
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (20) : 13324 - 13329
  • [4] Independent regulation of strigolactones and blumenols during arbuscular mycorrhizal symbiosis in rice
    Servante, Emily K.
    Halitschke, Rayko
    Rocha, Catarina
    Baldwin, Ian T.
    Paszkowski, Uta
    PLANT JOURNAL, 2024, 119 (03): : 1289 - 1298
  • [5] Plant phosphate transporter genes help harness the nutritional benefits of arbuscular, mycorrhizal symbiosis
    Smith, SE
    Barker, SJ
    TRENDS IN PLANT SCIENCE, 2002, 7 (05) : 189 - 190
  • [6] Characterization of a Lotus japonicus phosphate transporter (LjPT4) that is specific for arbuscular mycorrhizal symbiosis
    Maeda, D
    Ashida, K
    Iguchi, K
    Deguchi, Y
    Izui, K
    Hata, S
    BIOLOGICAL NITROGEN FIXATION, SUSTAINABLE AGRICULTURE AND THE ENVIRONMENT, 2005, 41 : 238 - 238
  • [7] The regulation of arbuscular mycorrhizal symbiosis by phosphate in pea involves early and systemic signalling events
    Balzergue, Coline
    Puech-Pages, Virginie
    Becard, Guillaume
    Rochange, Soizic F.
    JOURNAL OF EXPERIMENTAL BOTANY, 2011, 62 (03) : 1049 - 1060
  • [8] Multiple PHT1 family phosphate transporters are recruited for mycorrhizal symbiosis in Eucalyptus grandis and conserved PHT1;4 is a requirement for the arbuscular mycorrhizal symbiosis
    Che, Xianrong
    Lai, Wenzhen
    Wang, Sijia
    Wang, Xinyang
    Hu, Wentao
    Chen, Hui
    Xie, Xianan
    Tang, Ming
    TREE PHYSIOLOGY, 2022, 42 (10) : 2020 - 2039
  • [9] Positive Gene Regulation by a Natural Protective miRNA Enables Arbuscular Mycorrhizal Symbiosis
    Couzigou, Jean-Malo
    Lauressergues, Dominique
    Andre, Olivier
    Gutjahr, Caroline
    Guillotin, Bruno
    Becard, Guillaume
    Combier, Jean-Philippe
    CELL HOST & MICROBE, 2017, 21 (01) : 106 - 112
  • [10] Dynamics of Periarbuscular Membranes Visualized with a Fluorescent Phosphate Transporter in Arbuscular Mycorrhizal Roots of Rice
    Kobae, Yoshihiro
    Hata, Shingo
    PLANT AND CELL PHYSIOLOGY, 2010, 51 (03) : 341 - 353