The Schizosaccharomyces pombe spo3+ gene is required for assembly of the forespore membrane and genetically interacts with psy1+-encoding syntaxin-like protein

被引:120
作者
Nakamura, T
Nakamura-Kubo, M
Hirata, A
Shimoda, C [1 ]
机构
[1] Osaka City Univ, Grad Sch Sci, Dept Biol, Osaka 5588585, Japan
[2] Univ Tokyo, Grad Sch Frontier Sci, Dept Integrated Biosci, Chiba 2778562, Japan
关键词
D O I
10.1091/mbc.12.12.3955
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Formation of the forespore membrane, which becomes the plasma membrane of spores, is an intriguing step in the sporulation of the fission yeast Schizosaccharomyces pombe. Here we report two novel proteins that localize to the forespore membrane. spo3(+) encodes a potential membrane protein, which was expressed only during sporulation. Green fluorescent protein (GFP) fusion revealed that Spo3 localized to the forespore membrane. The spo3 disruptant was viable and executed meiotic nuclear divisions as efficiently as the wild type but did not form spores. One of the spo3 alleles, spo3-KC51, was dose-dependently suppressed by psy1(+), which encodes a protein similar to mammalian syntaxin-1A, a component of the plasma membrane docking/fusion complex. psy1(+) was essential for vegetative growth, and its transcription was enhanced during sporulation. As expected, Psy1 localized to the plasma membrane during vegetative growth. Interestingly, Psy1 on the plasma membrane disappeared immediately after first meiotic division and relocalized to the forespore membrane as the second division initiated. In the spo3 null mutant, the forespore membrane was initiated but failed to develop a normal morphology. Electron microscopy revealed that membrane vesicles were accumulated in the cytoplasm of immature spo3 Delta asci. These results suggest that Spo3 is a key component of the forespore membrane and is essential for its assembly acting in collaboration with the syntaxin-like protein.
引用
收藏
页码:3955 / 3972
页数:18
相关论文
共 52 条
[1]   YEAST SYNTAXINS SSO1P AND SSO2P BELONG TO A FAMILY OF RELATED MEMBRANE-PROTEINS THAT FUNCTION IN VESICULAR TRANSPORT [J].
AALTO, MK ;
RONNE, H ;
KERANEN, S .
EMBO JOURNAL, 1993, 12 (11) :4095-4104
[2]  
Abe H, 2000, GENETICS, V154, P1497
[3]   SPO21 is required for meiosis-specific modification of the spindle pole body in yeast [J].
Bajgier, BK ;
Malzone, M ;
Nickas, M ;
Neiman, AM .
MOLECULAR BIOLOGY OF THE CELL, 2001, 12 (06) :1611-1621
[4]   HIGH-FREQUENCY TRANSFORMATION OF THE FISSION YEAST SCHIZOSACCHAROMYCES-POMBE [J].
BEACH, D ;
NURSE, P .
NATURE, 1981, 290 (5802) :140-142
[5]   SYNTAXIN - A SYNAPTIC PROTEIN IMPLICATED IN DOCKING OF SYNAPTIC VESICLES AT PRESYNAPTIC ACTIVE ZONES [J].
BENNETT, MK ;
CALAKOS, N ;
SCHELLER, RH .
SCIENCE, 1992, 257 (5067) :255-259
[6]   SEC9 IS A SNAP-25-LIKE COMPONENT OF A YEAST SNARE COMPLEX THAT MAY BE THE EFFECTOR OF SEC4 FUNCTION IN EXOCYTOSIS [J].
BRENNWALD, P ;
KEARNS, B ;
CHAMPION, K ;
KERANEN, S ;
BANKAITIS, V ;
NOVICK, P .
CELL, 1994, 79 (02) :245-258
[7]   GENES INVOLVED IN MEIOSIS AND SPORULATION OF A YEAST [J].
BRESCH, C ;
MULLER, G ;
EGEL, R .
MOLECULAR AND GENERAL GENETICS, 1968, 102 (04) :301-&
[8]   The synaptic protein syntaxin 1 is required for cellularization of Drosophila embryos [J].
Burgess, RW ;
Deitcher, DL ;
Schwarz, TL .
JOURNAL OF CELL BIOLOGY, 1997, 138 (04) :861-875
[9]   PREMEIOTIC DNA-SYNTHESIS IN FISSION YEAST [J].
EGEL, R ;
EGELMITA.M .
EXPERIMENTAL CELL RESEARCH, 1974, 88 (01) :127-134
[10]   General purpose tagging vectors for fission yeast [J].
Forsburg, SL ;
Sherman, DA .
GENE, 1997, 191 (02) :191-195