Characterization of a cartilage-derived 66-kDa protein (RGD-CAP/beta ig-h3) that binds to collagen

被引：144
作者：
Hashimoto, K
Noshiro, M
Ohno, S
Kawamoto, T
Satakeda, H
Akagawa, Y
Nakashima, K
Okimura, A
Ishida, H
Okamoto, T
Pan, H
Shen, M
Yan, WQ
Kato, Y
机构：
[1] HIROSHIMA UNIV,SCH DENT,DEPT BIOCHEM,MINAMI KU,HIROSHIMA 734,JAPAN
[2] HIROSHIMA UNIV,SCH DENT,DEPT REMOVAL PROSTHODONT,MINAMI KU,HIROSHIMA 734,JAPAN
[3] HIROSHIMA UNIV,SCH DENT,DEPT ORTHODONT,HIROSHIMA 734,JAPAN
[4] HIROSHIMA UNIV,SCH DENT,DEPT ORAL & MAXILLOFACIAL SURG 1,MINAMI KU,HIROSHIMA 734,JAPAN
[5] NIPPON HOECHST MARION ROUSSEL,LAB BONE RES,KAWAGOE,SAITAMA 350,JAPAN
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1997年 / 1355卷 / 03期
关键词：
collagen-binding protein; cartilage; chondrocyte; collagen; RGD; TGF-beta;
D O I：
10.1016/S0167-4889(96)00147-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A 66-kDa collagen fiber-associated protein (RGD-CAP) was isolated from a fiber-rich fraction of pig cartilage by ultrafiltration and collagen-affinity chromatography. Amino acid sequencing and cDNA cloning indicated that the RGD-CAP is identical or closely related to beta ig-h3 protein which is induced in human adenocarcinoma cells by transforming growth factor-beta (TGF-beta) (Skonier, J., Neubauer, M., Madisen, L., Bennett, K., Plowman, G.D., and Purchio, A.F. (1992) DNA Cell. Biol. 11, 511-522). The RGD-CAP, as well as beta ig-h3, has the RGD sequence in the C-terminal region. The native RGD-CAP bound to type I, II, and IV collagens even in the presence of 1 M NaCl. A recombinant preparation of RGD-CAP expressed in Escherichia coli cells also bound to collagen but not to gelatin. The RGD-CAP mRNA was expressed in chondrocytes throughout all stages, although the expression level was highest during the prehypertrophic stage. In addition, TGF-beta increased the RGD-CAP mRNA level in chondrocyte cultures. Since RGD-CAP transcripts were found in most tissues, this novel collagen-binding protein may play an important role in cell-collagen interactions in various tissues including developing cartilage.
引用
收藏
页码：303 / 314
页数：12
相关论文
共 39 条
[1]  
BROWN RA, 1990, RHEUMATOLOGY, V17, P65
[2]   DROSOPHILA FASCICLIN-I IS A NOVEL HOMOPHILIC ADHESION MOLECULE THAT ALONG WITH FASCICLIN-III CAN MEDIATE CELL SORTING [J].
ELKINS, T ;
HORTSCH, M ;
BIEBER, AJ ;
SNOW, PM ;
GOODMAN, CS .
JOURNAL OF CELL BIOLOGY, 1990, 110 (05) :1825-1832
[3]  
ERCOLANI L, 1988, J BIOL CHEM, V263, P15335
[4]   CDNA FROM HUMAN OCULAR CILIARY EPITHELIUM HOMOLOGOUS TO BETA-IG-H3 IS PREFERENTIALLY EXPRESSED AS AN EXTRACELLULAR PROTEIN IN THE CORNEAL EPITHELIUM [J].
ESCRIBANO, J ;
HERNANDO, N ;
GHOSH, S ;
CRABB, J ;
COCAPRADOS, M .
JOURNAL OF CELLULAR PHYSIOLOGY, 1994, 160 (03) :511-521
[5]   REDUCED AMOUNTS OF CARTILAGE COLLAGEN FIBRILS AND GROWTH PLATE ANOMALIES IN TRANSGENIC MICE HARBORING A GLYCINE-TO-CYSTEINE MUTATION IN THE MOUSE TYPE-II PROCOLLAGEN ALPHA-1-CHAIN GENE [J].
GAROFALO, S ;
VUORIO, E ;
METSARANTA, M ;
ROSATI, R ;
TOMAN, D ;
VAUGHAN, J ;
LOZANO, G ;
MAYNE, R ;
ELLARD, J ;
HORTON, W ;
DECROMBRUGGHE, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (21) :9648-9652
[6]   ANALYSIS OF ALPHA(1)BETA(1), ALPHA(2)BETA(1) AND ALPHA(3)BETA(1) INTEGRINS IN CELL-COLLAGEN INTERACTIONS - IDENTIFICATION OF CONFORMATION DEPENDENT ALPHA(1)BETA(1) BINDING-SITES IN COLLAGEN TYPE-I [J].
GULLBERG, D ;
GEHLSEN, KR ;
TURNER, DC ;
AHLEN, K ;
ZIJENAH, LS ;
BARNES, MJ ;
RUBIN, K .
EMBO JOURNAL, 1992, 11 (11) :3865-3873
[7]  
HASCALL VC, 1974, J BIOL CHEM, V249, P4242
[8]  
IWAMOTO M, 1991, J BIOL CHEM, V266, P461
[9]   CHARACTERIZATION OF THE MINERALIZATION PROCESS IN CULTURES OF RABBIT GROWTH PLATE CHONDROCYTES [J].
JIKKO, A ;
AOBA, T ;
MURAKAMI, H ;
TAKANO, Y ;
IWAMOTO, M ;
KATO, Y .
DEVELOPMENTAL BIOLOGY, 1993, 156 (02) :372-380
[10]   TERMINAL DIFFERENTIATION AND CALCIFICATION IN RABBIT CHONDROCYTE CULTURES GROWN IN CENTRIFUGE TUBES - REGULATION BY TRANSFORMING GROWTH FACTOR-BETA AND SERUM FACTORS [J].
KATO, Y ;
IWAMOTO, M ;
KOIKE, T ;
SUZUKI, F ;
TAKANO, Y .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (24) :9552-9556
1234