Fluorinated Bambusurils as Highly Effective and Selective Transmembrane Cl-/HCO3- Antiporters

被引:67
|
作者
Valkenier, Hennie [1 ]
Akrawi, Omer [2 ,3 ]
Jurcek, Pia [2 ,3 ]
Sleziakova, Kristina [2 ,3 ]
Lizal, Tomas [2 ,3 ]
Bartik, Kristin [1 ]
Sindelar, Vladimir [2 ,3 ]
机构
[1] Univ Libre Bruxelles, Ecole Polytech Bruxelles, Engn Mol NanoSyst, Ave FD Roosevelt 50,CP165-64, B-1050 Brussels, Belgium
[2] Masaryk Univ, Dept Chem, Kamenice 5, Brno 62500, Czech Republic
[3] Masaryk Univ, RECETOX, Kamenice 5, Brno 62500, Czech Republic
来源
CHEM | 2019年 / 5卷 / 02期
关键词
ANION TRANSPORT; MEDIATED TRANSPORT; CHLORIDE TRANSPORT; HIGH-AFFINITY; BICARBONATE; MEMBRANE; BINDING; POWERFUL; NITRATE; MACROCYCLE;
D O I
10.1016/j.chempr.2018.11.008
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The exchange of chloride and bicarbonate across lipid bilayers is an important biological process. Synthetic molecules can act as mobile carriers for these anions, although most show little selectivity. Here we report on three bambus[6] uril macrocycles functionalized with fluorinated benzyl groups, which are able to exchange Cl- and HCO3- efficiently. Remarkably, rates for Cl-/NO3- exchange are two orders of magnitude lower. The higher rates of a Cl-/HCO3- transport can be explained by the ability of the bambusurils to complex Cl- and HCO3- simultaneously, facilitating their exchange at the bilayer interface. Furthermore, the exceptionally high affinity and selectivity of these systems for NO3- appear to contribute to the poor Cl-/NO3- exchange. This work not only demonstrates the importance of anion binding characteristics on anion transport but also the potential relevance of bambusurils for anion transport applications considering the high rate observed for Cl-/HCO3- exchange.
引用
收藏
页码:429 / 444
页数:16
相关论文
共 50 条
  • [1] Bestrophin Cl- channels are highly permeable to HCO3-
    Qu, Zhiqiang
    Hartzell, H. Criss
    AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2008, 294 (06): : C1371 - C1377
  • [2] Independence of apical Cl-/HCO3- exchange and anion conductance in duodenal HCO3- secretion
    Spiegel, S
    Phillipper, M
    Rossmann, H
    Riederer, B
    Gregor, M
    Seidler, U
    AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2003, 285 (05): : G887 - G897
  • [3] HCO3- ABSORPTION BY APICAL NA+/H+ AND CL-/HCO3- EXCHANGES
    STEPHENSON, RL
    FELDMAN, GM
    KIDNEY INTERNATIONAL, 1990, 37 (01) : 546 - 546
  • [4] NA-DEPENDENT HCO3- TRANSPORT AND CL-/HCO3- EXCHANGE IN CILIARY EPITHELIUM
    WOLOSIN, JM
    BONANNO, JA
    MACHEN, TE
    ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1989, 574 : 131 - 133
  • [5] NA-DEPENDENT HCO3- TRANSPORT AND CL-/HCO3- EXCHANGE IN CILIARY EPITHELIUM
    WOLOSIN, JM
    BONANNO, JA
    MACHEN, TE
    BICARBONATE, CHLORIDE, AND PROTON TRANSPORT SYSTEMS, 1989, 574 : 131 - 133
  • [6] Cl- is required for HCO3- entry necessary for sperm capacitation:involvement of a Cl-/HCO3- exchanger(SLC26A3) and CFTR
    Chen, Wen-Ying
    Xu, Wen-Ming
    Chen, Zhang-Hui
    Ni, Ya
    Yuan, Yu-Ying
    Zhou, Si-Chang
    Li, Kun
    Tsang, L. L.
    Chung, Yin Wa
    Shi, Qi-Xian
    Chan, Hsiao Chang
    BIOLOGY OF REPRODUCTION, 2008, : 187 - 187
  • [7] Negative News: Cl- and HCO3- in the Vascular Wall
    Boedtkjer, Ebbe
    Matchkov, Vladimir V.
    Boedtkjer, Donna M. B.
    Aalkjaer, Christian
    PHYSIOLOGY, 2016, 31 (05) : 370 - 383
  • [8] Regulation of intestinal Cl- and HCO3- secretion by uroguanylin
    Joo, NS
    London, RM
    Kim, HD
    Forte, LR
    Clarke, LL
    AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 1998, 274 (04): : G633 - G644
  • [9] The AE gene family of Cl-/HCO3- exchangers
    Alper, SL
    Darman, RB
    Chernova, MN
    Dahl, NK
    JOURNAL OF NEPHROLOGY, 2002, 15 : S41 - S53
  • [10] ISOLATED INTRAHEPATIC BILE-DUCT UNITS SECRETE CL- AND HCO3- AND EXPRESS THE MESSENGER-RNA FOR A CL-/HCO3- EXCHANGER
    ROBERTS, S
    PHAM, L
    BALAN, V
    KUNTZ, S
    ALPINI, G
    GORES, G
    LARUSSO, N
    HEPATOLOGY, 1993, 18 (04) : A144 - A144