Fine mapping of two major QTLs conferring resistance to powdery mildew in tomato

被引：10
作者：
Faino, Luigi [1 ,2 ]
Azizinia, Shiva [1 ,3 ]
Hassanzadeh, Benyamin Houshyani [1 ]
Verzaux, Estelle [1 ]
Ercolano, Maria Raffaella [2 ]
Visser, Richard G. F. [1 ]
Bai, Yuling [1 ]
机构：
[1] Univ Wageningen & Res Ctr, Wageningen UR Plant Breeding, NL-6708 PB Wageningen, Netherlands
[2] Univ Naples Federico II, Dept Soil Plant Environm & Anim Prod Sci, I-80055 Portici, Italy
[3] Univ Tehran, Dept Hort, Coll Abouraihan, Tehran, Iran
来源：
EUPHYTICA | 2012年 / 184卷 / 02期
关键词：
Quantitative resistance loci (QRLs); Powdery mildew; Recombinant families; Fine mapping; QUANTITATIVE TRAIT LOCI; DISEASE RESISTANCE; OIDIUM-NEOLYCOPERSICI; PHYTOPHTHORA-INFESTANS; DEPENDENT RESISTANCE; PLANT DEFENSE; GENETIC-MAP; LATE BLIGHT; LINKAGE; POTATO;
D O I：
10.1007/s10681-011-0551-6
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Tomato (Solanum lycopersicum) is the most cultivated crop in the Solanaceae family and is a host for Oidium neolycopersici, the cause agent of powdery mildew disease. In wild species of tomato, genes (Ol-1-Ol-6) for monogenic resistance have been identified. Moreover, three quantitative resistance loci (QRLs), namely Ol-qtl1, Ol-qtl2 and Ol-qtl3, have been mapped in Solanum neorickii G1.1601. In this work, we developed several advanced backcross populations in order to fine-map these Ol-qtls. Resistant lines harboring individual Ol-qtl were produced and used in recombinant screening. Ten recombinants were identified in chromosomal regions carrying Ol-qtl1s. The recombinant individuals were used to produce recombinant families (RFs). By screening these RFs with molecular markers and testing them with O. neolycopersici, we could localize Ol-qtl1 in a region of about 2.3 Mbp on the long arm of chromosome 6 and Ol-qtl2 in a region of 2.5 Mbp on the short arm of chromosome 12. On the other hand, the presence of Ol-qtl3 locus was not confirmed in this study. The fine-mapping results further demonstrated the co-localization between Ol-qtls and genes for monogenic resistance; the Ol-qtl1 interval contains the Ol-1 gene and the Ol-qtl2 interval harbors the Lv gene that confers monogenic resistance to Leveillula taurica, another species of tomato powdery mildew.
引用
收藏
页码：223 / 234
页数：12
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