β-Asarone Exerts Antioxidative Effects on H2O2-Stimulated PC12 Cells by Activating Nrf2/HO-1 Pathway

被引：19

作者：

Hei, Xinxin ^{[1
,2
]}

Xie, Miao ^{[3
]}

Xu, Jingqian ^{[1
]}

Li, Jinjin ^{[1
]}

Liu, Tao ^{[1
]}

机构：

[1] Nanjing Univ Chinese Med, Coll Tradit Chinese Med, Coll Integrated Chinese & Western Med, Nanjing, Peoples R China

[2] Liyang City Hosp TCM, Changzhou, Peoples R China

[3] Henan Univ Chinese Med, Coll Basic Med, Zhengzhou, Peoples R China

来源：

NEUROCHEMICAL RESEARCH | 2020年 / 45卷 / 08期

关键词：

beta-Asarone; Neurotoxicity; Oxidative stress; Nrf2/HO-1; OXIDATIVE STRESS; NRF2; DISEASES; MEMORY; RATS;

D O I：

10.1007/s11064-020-03060-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oxidative stress-mediated neuron damage is considered an important contributor to the pathogenesis and development of neurodegenerative diseases. Although beta-asarone is widely known for its neuroprotective pharmacological properties, the exact mechanism of beta-asarone against oxidative stress has not been fully elucidated. The aim of the present study was to investigate underlying mechanisms of beta-asarone against oxidative damage in PC12 cells. Our results demonstrated that the treatment of beta-asarone significantly alleviated the reduction in cell viability and the excessive accumulation of lactate dehydrogenase (LDH), malondialdehyde (MDA) and reactive oxygen species (ROS) by increasing the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH). Moreover, beta-asarone pretreatment also activated nuclear factor 2 erythroid-related factor 2 (Nrf2) and its downstream target heme oxygenase-1 (HO-1), which was involved in quenching reactive oxygen to inhibit oxidative stress. Furthermore, when silenced by Nrf2 siRNA, the protective effect of beta-asarone was reduced and the oxidative stress induced by H2O2 was enhanced. In conclusion, our findings revealed that beta-asarone could reduce oxidative stress via activating Nrf2/HO-1 pathway in PC12 cells, highlighting the potential therapeutic role of beta-asarone in neurodegenerative diseases.

引用

页码：1953 / 1961

页数：9

共 40 条

[1] Oxidative stress, mitochondrial dysfunction and neurodegenerative diseases; a mechanistic insight [J].

Bhat, Aashiq Hussain ;

Dar, Khalid Bashir ;

Anees, Suhail ;

Zargar, Mohammad Afzal ;

Masood, Akbar ;

Sofi, Manzoor Ahmad ;

Ganie, Showkat Ahmad .

BIOMEDICINE & PHARMACOTHERAPY, 2015, 74 :101-110

[2] Nrf2-ARE pathway: An emerging target against oxidative stress and neuroinflammation in neurodegenerative diseases [J].

Buendia, Izaskun ;

Michalska, Patrycja ;

Navarro, Elisa ;

Gameiro, Isabel ;

Egea, Javier ;

Leon, Rafael .

PHARMACOLOGY & THERAPEUTICS, 2016, 157 :84-104

[3] Oxidative Stress and Antioxidants in Neurological Diseases: Is There Still Hope? [J].

Carvalho, Andreia Neves ;

Firuzi, Omidreza ;

Gama, Maria Joao ;

van Horssen, Jack ;

Saso, Luciano .

CURRENT DRUG TARGETS, 2017, 18 (06) :705-718

[4] Combined application of tenuigenin and β-asarone improved the efficacy of memantine in treating moderate-to-severe Alzheimer's disease [J].

Chang, Wenguang ;

Teng, Junfang .

DRUG DESIGN DEVELOPMENT AND THERAPY, 2018, 12 :455-462

[5] Pharmacology and toxicology of α- and β-Asarone: A review of preclinical evidence [J].

Chellian, Ranjithkumar ;

Pandy, Vijayapandi ;

Mohamed, Zahurin .

PHYTOMEDICINE, 2017, 32 :41-58

[6] Nrf2 at the heart of oxidative stress and cardiac protection [J].

Chen, Qin M. ;

Maltagliati, Anthony J. .

PHYSIOLOGICAL GENOMICS, 2018, 50 (02) :77-97

[7] Uncoupling neuronal death and dysfunction in Drosophila models of neurodegenerative disease [J].

Chouhan, Amit K. ;

Guo, Caiwei ;

Hsieh, Yi-Chen ;

Ye, Hui ;

Senturk, Mumine ;

Zuo, Zhongyuan ;

Li, Yarong ;

Chatterjee, Shreyasi ;

Botas, Juan ;

Jackson, George R. ;

Bellen, Hugo J. ;

Shulman, Joshua M. .

ACTA NEUROPATHOLOGICA COMMUNICATIONS, 2016, 4 :62

[8] Activation of anti-oxidant Nrf2 signaling by substituted trans stilbenes [J].

Deck, Lorraine M. ;

Whalen, Lisa J. ;

Hunsaker, Lucy A. ;

Royer, Robert E. ;

Vander Jagt, David L. .

BIOORGANIC & MEDICINAL CHEMISTRY, 2017, 25 (04) :1423-1430

[9] β-asarone improves learning and memory and reduces Acetyl Cholinesterase and Beta-amyloid 42 levels in APP/PS1 transgenic mice by regulating Beclin-1-dependent autophagy [J].

Deng, Minzhen ;

Huang, Liping ;

Ning, Baile ;

Wang, Nanbu ;

Zhang, Qinxin ;

Zhu, Caixia ;

Fang, Yongqi .

BRAIN RESEARCH, 2016, 1652 :188-194

[10] ALS-FTLD-linked mutations of SQSTM1/p62 disrupt selective autophagy and NFE2L2/NRF2 anti-oxidative stress pathway [J].

Deng, Zhiqiang ;

Lim, Junghyun ;

Wang, Qian ;

Purtell, Kerry ;

Wu, Shuai ;

Palomo, Gloria M. ;

Tan, Haiyan ;

Manfredi, Giovanni ;

Zhao, Yanxiang ;

Peng, Junmin ;

Hu, Bo ;

Chen, Shi ;

Yue, Zhenyu .

AUTOPHAGY, 2020, 16 (05) :917-931

← 1 2 3 4 →