Isolation of viable egg cells of rape (Brassica napus L)

被引：15

作者：

Katoh, N ^{[1
]}

Lorz, H ^{[1
]}

Kranz, E ^{[1
]}

机构：

[1] JAPAN TOBACCO INC,APPL PLANT RES LAB,OYAMA,JAPAN

来源：

ZYGOTE | 1997年 / 5卷 / 01期

关键词：

egg cell isolation; embryo sac; Brassica;

D O I：

10.1017/S0967199400003531

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The isolation of viable egg cells of rape (Brassica napus L.) has been achieved from microdissected ovules. The non-gametic cells of the embryo sac, synergids and central cells have also been isolated. Their morphology corresponded to that of these cells in situ, making a discrimination from isolated sporophytic cells possible. Two hours after isolation the egg cells were still viable. Viable egg cells have been reproducibly isolated with a frequency of 25% per dissected ovule.

引用

页码：31 / &

页数：4

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