Spatially Resolved Recording of Transient Fluorescence-Lifetime Effects by Line-Scanning TCSPC

被引：17

作者：

Becker, Wolfgang ^{[1
]}

Shcheslavkiy, Vladislav ^{[1
]}

Frere, Samuel ^{[2
]}

Slutsky, Inna ^{[2
]}

机构：

[1] Becker & Hickl GmbH, D-12277 Berlin, Germany

[2] Tel Aviv Univ, Sackler Fac Med, Dept Physiol & Pharmacol, Sagol Sch Neurosci, IL-69978 Tel Aviv, Israel

来源：

MICROSCOPY RESEARCH AND TECHNIQUE | 2014年 / 77卷 / 03期

关键词：

Chlorophyll transients; FLIM; FLITS; Fluorescence lifetime; TCSPC;

D O I：

10.1002/jemt.22331

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

We present a technique that records transient changes in the fluorescence lifetime of a sample with spatial resolution along a one-dimensional scan. The technique is based on scanning the sample with a high-frequency pulsed laser beam, detecting single photons of the fluorescence light, and building up a photon distribution over the distance along the scan, the arrival times of the photons after the excitation pulses and the time after a stimulation of the sample. The maximum resolution at which lifetime changes can be recorded is given by the line scan period. Transient lifetime effects can thus be resolved at a resolution of about one millisecond. We demonstrate the technique for recording photochemical and nonphotochemical chlorophyll transients in plants and transient changes in free Ca2+ in cultured neurons. Microsc. Res. Tech. 77:216-224, 2014. © 2014 Wiley Periodicals, Inc.

引用

页码：216 / 224

页数：9

共 24 条

[21] NMDA receptors, place cells and hippocampal spatial memory [J].