Revolutionizing immunology with single-cell RNA sequencing

被引：144

作者：

Chen, Haide ^{[1
,2
,3
]}

Ye, Fang ^{[1
]}

Guo, Guoji ^{[1
,2
,3
,4
,5
]}

机构：

[1] Zhejiang Univ, Ctr Stem Cell & Regenerat Med, Sch Med, Hangzhou 310058, Zhejiang, Peoples R China

[2] Zhejiang Univ, Affiliated Hosp 1, Bone Marrow Transplantat Ctr, Sch Med, Hangzhou 310009, Zhejiang, Peoples R China

[3] Dr Li Dak Sum & Yip Yio Chin Ctr Stem Cell & Rege, Zhejiang Prov Key Lab Tissue Engn & Regenerat Med, Hangzhou 310058, Zhejiang, Peoples R China

[4] Zhejiang Univ, Inst Hematol, Hangzhou 310058, Zhejiang, Peoples R China

[5] Zhejiang Univ, Stem Cell Inst, Hangzhou 310058, Zhejiang, Peoples R China

来源：

CELLULAR & MOLECULAR IMMUNOLOGY | 2019年 / 16卷 / 03期

关键词：

INNATE LYMPHOID-CELLS; HEMATOPOIETIC STEM; LINEAGE COMMITMENT; TRANSCRIPTOME; SEQ; PROGENITOR; HETEROGENEITY; LANDSCAPE; REVEALS; VARIABILITY;

D O I：

10.1038/s41423-019-0214-4

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The immune system is composed of a complex hierarchy of cell types that protect the organism against disease and maintain homeostasis. Identifying heterogeneity of immune cells is the key to understanding the immune system. Advanced single-cell RNA sequencing (scRNA-seq) technologies are revolutionizing our ability to study immunology. By measuring transcriptomes at the single-cell level, scRNA-seq enables identification of cellular heterogeneity in far greater detail than conventional methods. In this review, we introduce the existing scRNA-seq technologies and present their strengths and weaknesses. We also discuss potential applications and future innovations of scRNA-seq in immunology.

引用

页码：242 / 249

页数：8

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