Stimulative effects of light and a temperature downshift on transcriptional expressions of developmentally regulated genes in the initial stages of fruiting-body formation of the basidiomycetous mushroom Lentinula edodes

被引:18
作者
Nakazawa, Takehito [1 ]
Miyazaki, Yasumasa [2 ]
Kaneko, Shinya [1 ]
Shishido, Kazuo [1 ]
机构
[1] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Life Sci, Midori Ku, Kanagawa 2268501, Japan
[2] Forestry & Forest Prod Res Inst, Dept Appl Microbiol, Tsukuba, Ibaraki, Japan
关键词
developmentally regulated genes; reverse-transcription PCR; environmental factors; fruiting-body formation; Lentinula edodes; mushroom;
D O I
10.1111/j.1574-6968.2008.01364.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Quantitative reverse transcription PCR experiments were done to analyze the effects of light and a temperature downshift on transcriptional expressions of a variety of developmentally regulated genes in the initial stages of fruiting-body formation of Lentinula edodes wild-type strain FMC2. It was revealed that both proper light conditions and a temperature downshift are required for high levels of transcriptions of the genes and the formation of fruiting bodies. In L. edodes MIL-LEW-M13-1 strain, which has been reported previously to be capable of forming fruiting bodies without a low-temperature treatment, the expressions of the developmentally regulated genes were enhanced by light exposure.
引用
收藏
页码:67 / 71
页数:5
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