Investigating glucose and xylose metabolism in Saccharomyces cerevisiae and Scheffersomyces stipitis via 13C metabolic flux analysis

被引：19

作者：

Feng, Xueyang ^{[1
]}

Zhao, Huimin ^{[1
,2
]}

机构：

[1] Univ Illinois, Inst Genom Biol, Dept Chem & Biomol Engn, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Chem Biochem & Bioengn, Inst Genom Biol, Urbana, IL 61801 USA

来源：

AICHE JOURNAL | 2013年 / 59卷 / 09期

关键词：

(13)CMFA; glucose metabolism; xylose metabolism; mixed sugar; yeasts; TCA cycle; PARALLEL LABELING EXPERIMENTS; ENERGY GENERATION; PICHIA-PASTORIS; YEAST; GROWTH; COLI;

D O I：

10.1002/aic.14182

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

C-13 metabolic flux analysis ((13)CMFA) has been extensively applied in studying the glucose metabolism of yeast strains such as Saccharomyces cerevisiae and Scheffersomyces stipitis. Here, we tried to augment the previous fluxomic studies by applying (13)CMFA to rigorously investigate the metabolic flux distributions in S. stipitis and the recombinant S. cerevisiae strains when xylose was utilized as the sole carbon substrate. It was found that less carbon fluxes were diverted into the TCA cycle in S. stipitis than the recombinant S. cerevisiae strains. Compared to single sugar utilization, the co-utilization of glucose and xylose by S. stipitis led to increased metabolic fluxes into the futile pathway and the TCA cycle, but did not improve sugar-based ethanol yield. In addition, heterologous expression of xylose pathway in engineered S. cerevisiae strains may affect the glucose utilization. (c) 2013 American Institute of Chemical Engineers AIChE J, 59: 3195-3202, 2013

引用

页码：3195 / 3202

页数：8

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