Bilayer electrospun poly(vinyl alcohol)-gelatin mat and biphasic calcium phosphate-pectin-gelatin hydrogel for application in bone hemorrhage

被引：1

作者：

Nguyen Thuy Ba Linh ^{[1
,2
]}

Padalhin, Andrew R. ^{[1
]}

Lee, Byung-Yeol ^{[3
]}

Lee, Byong-Taek ^{[1
,2
]}

机构：

[1] Soonchunhyang Univ, Dept Regenerat Med, Coll Med, Cheonan 330721, South Korea

[2] Soonchunhyang Univ, Inst Tissue Regenerat, Coll Med, Cheonan 330721, South Korea

[3] Soonchunhyang Univ, InoBone Corp R&D Ctr, Asan, South Korea

来源：

JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS | 2015年 / 30卷 / 04期

关键词：

Bilayer hemostatic scaffold; biphasic calcium phosphate-pectin-gelatin; electrospun poly(vinyl alcohol)-gelatin; bone hemorrhage application; hemostatic agent; TOPICAL HEMOSTATIC AGENTS; LOCAL AGENTS; FABRICATION; SURGICEL; COLLAGEN; POLYMER; WAX;

D O I：

10.1177/0883911515578762

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A bilayer composite scaffold consisting of a biphasic calcium phosphate-pectin-gelatin hydrogel and an electrospun poly(vinyl alcohol)-gelatin matrix was developed and investigated for application in bone hemorrhage. The aim of this research is to develop a new biomaterial system that provides a hemostatic effect on bone hemorrhage and does not interfere with native bone regeneration. The role of electrospun poly(vinyl alcohol)-gelatin in scaffolds is to provide the covering of the wound, while encapsulation and absorption of red blood cells are attributed to the biphasic calcium phosphate-pectin-gelatin hydrogel. Cell viability and cell proliferation were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Live/Dead assay, and immunofluorescence assay using pre-osteoblast MC3T3-E1 cells. The effects with and without hemostatic bilayer scaffolds on rat calvaria were compared at 1 and 3weeks. The hemostatic bilayer agent showed good blood absorption behavior and efficient bone healing properties after 3weeks, as observed by micro-computed tomography and hematoxylin and eosin staining.

引用

页码：424 / 435

页数：12

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