An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection

被引：30

作者：

Mayorga-Martinez, Carmen C. ^{[1
]}

Chamorro-Garcia, Alejandro ^{[1
,2
]}

Serrano, Lorena ^{[3
]}

Rivas, Lourdes ^{[1
,2
]}

Quesada-Gonzalez, Daniel ^{[1
,2
]}

Altet, Laura ^{[3
]}

Francino, Olga ^{[2
,3
]}

Sanchez, Armand ^{[3
,5
]}

Merkoci, Arben ^{[1
,4
]}

机构：

[1] Inst Catala Nanociencia & Nanotecnol ICN2, Nanobioelect & Biosensors Grp, Bellaterra 08193, Barcelona, Spain

[2] Autonomous Univ Barcelona, Bellaterra 08193, Spain

[3] Vetgenomics, Bellaterra 08193, Barcelona, Spain

[4] ICREA, Barcelona 08010, Spain

[5] Ctr Res Agr Genom CSIC IRTA UAB UB, Cerdanyola Del Valles 08193, Catalonia, Spain

来源：

JOURNAL OF MATERIALS CHEMISTRY B | 2015年 / 3卷 / 26期

关键词：

ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY; WATER OXIDATION; CARBON NANOTUBE; BIOSENSORS; POLY(THIONINE); REAL;

D O I：

10.1039/c5tb00545k

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

An impedimetric label-free genosensor for high sensitive DNA detection is developed. This system is based on a screen-printed carbon electrode modified with the thionine layer and iridium oxide nanoparticles (IrO2 NP). An aminated oligonucleotide probe is immobilized on the IrO2 NP/polythionine modified electrode and ethanolamine was used as a blocking agent. Different diluted PCR amplified DNA samples have been detected. The selectivity and reproducibility of this system are studied and the system was highly reproducible with RSD approximate to 15% and sensitive enough while using 2% of ethanolamine during the blocking step employed for genosensor preparation.

引用

页码：5166 / 5171

页数：6

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