Isolation and characterization of a new Na+/H+ antiporter gene OsNHA1 from rice (Oryza sativa L.)

被引：20

作者：

Zhou, Guo-An ^{[1
]}

Jiang, Yan ^{[1
]}

Yang, Qing ^{[1
]}

Wang, Jian-Fei ^{[1
]}

Huang, Ji ^{[1
]}

Zhang, Hong-Sheng ^{[1
]}

机构：

[1] Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China

来源：

DNA SEQUENCE | 2006年 / 17卷 / 01期

关键词：

rice; Na+/H+ antiporter; gene cloning and expression; salt stress;

D O I：

10.1080/10425170500224263

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The full-length cDNA (3612 bp) of OsNHA1 was cloned by RT-PCR approach from rice (Oryza sativa L.), which encodes a putative plasma membrane Na+/H+ antiporter. Its deduced protein, OsNHA1, has 11 transmembrane domains and a significant similarity to a plasma membrane Na+/H+ antiporter AtNHA1 from Arabidopsis thaliana. Phylogenetic analysis showed that the OsNHA1 clusters with the plasma membrane Na+/H+ antiporters from various organisms. The semi-quantitative RT-PCR assay revealed that the expression of OsNHA1 was up-regulated in both shoots and roots of rice seedlings under salt stress, whereas it was not induced in the rice seedlings treated by drought stress.

引用

页码：24 / 30

页数：7

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