Eosinophils from Murine Lamina Propria Induce Differentiation of Naive T Cells into Regulatory T Cells via TGF-β1 and Retinoic Acid

被引：19
作者：
Chen, Hong-Hu [1 ,2 ,3 ,4 ]
Sun, Ai-Hua [5 ]
Ojcius, David M. [6 ]
Hu, Wei-Lin [1 ,2 ,3 ]
Ge, Yu-Mei [2 ,3 ]
Lin, Xu'ai [1 ,2 ,3 ]
Li, Lan-Juan [1 ,2 ]
Pan, Jian-Ping [7 ]
Yan, Jie [1 ,2 ,3 ]
机构：
[1] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou 310003, Zhejiang, Peoples R China
[2] Zhejiang Univ, Sch Med, State Key Lab Diag & Treatment Infect Dis, Div Basic Med Microbiol,Affiliated Hosp 1, Hangzhou 310058, Zhejiang, Peoples R China
[3] Zhejiang Univ, Dept Med Microbiol & Parasitol, Sch Med, Hangzhou 310058, Zhejiang, Peoples R China
[4] Zhejiang Prov Ctr Dis Control & Prevent, Hangzhou 310051, Zhejiang, Peoples R China
[5] Zhejiang Med Coll, Fac Basic Med, Hangzhou 310053, Zhejiang, Peoples R China
[6] Univ Calif, Hlth Sci Res Inst & Mol Cell Biol, Merced, CA 95343 USA
[7] Zhejiang Univ City Coll, Sch Med, Dept Clin Med, Hangzhou 310015, Zhejiang, Peoples R China
来源：
PLOS ONE | 2015年 / 10卷 / 11期
基金：
中国国家自然科学基金;
关键词：
DENDRITIC CELLS; REG-CELLS; GENERATION; FOXP3; QUANTIFICATION; INTESTINE; RESPONSES; T(H)17; HEALTH;
D O I：
10.1371/journal.pone.0142881
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Treg cells play a crucial role in immune tolerance, but mechanisms that induce Treg cells are poorly understood. We here have described eosinophils in lamina propria (LP) that displayed high aldehyde dehydrogenase (ALDH) activity, a rate-limiting step during all-trans retinoic acid (ATRA) synthesis, and expressed TGF-beta 1 mRNA and high levels of ATRA. Co-incubation assay confirmed that LP eosinophils induced the differentiation of naive T cells into Treg cells. Differentiation promoted by LP eosinophils were inhibited by blocked either TGF-beta 1 or ATRA. Peripheral blood (PB) eosinophils did not produce ATRA and could not induce Treg differentiation. These data identifies LP eosinophils as effective inducers of Treg cell differentiation through a mechanism dependent on TGF-beta 1 and ATRA.
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页数：13
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