Trypanosoma evansi: Detection of Trypanosoma evansi DNA in naturally and experimentally infected animals using TBR1 & TBR2 primers

被引：13

作者：

Ashour, Ameen A. ^{[1
]}

Abou El-Naga, Tarek R. ^{[2
]}

Barghash, Safaa M. ^{[2
]}

Salama, Mohamed S. ^{[3
]}

机构：

[1] Ain Shams Univ, Fac Sci, Dept Zool, Cairo, Egypt

[2] Desert Res Ctr, Dept Anim Hlth, Cairo, Egypt

[3] Ain Shams Univ, Fac Sci, Dept Entomol, Cairo, Egypt

来源：

EXPERIMENTAL PARASITOLOGY | 2013年 / 134卷 / 01期

关键词：

Trypanozoon; Trypanosoma evansi; PCR: polymerase chain reaction; TBR; 1/2; Egypt; PCR; AMPLIFICATION; EQUIPERDUM; STOCKS;

D O I：

10.1016/j.exppara.2013.02.003

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A polymerase chain reaction (PCR-based) assay was evaluated for detection of Trypanosoma evansi DNA in experimentally infected mice and naturally infected camels, sheep and goats using the set of primers TBr1 & TBr2 that amplified 164 bp DNA fragment. The results revealed that PCR-based assay was able to detect T. evansi directly from the blood during both acute and chronic phase of infection in all tested animals and in the blood and tissues of intraperitoneally infected mice depending upon the level of infection in the test samples. PCR was more powerful than CATT/T. evansi and mouse inoculation tests, when detected the infection in mice (24 h) post infection. Present results show that sheep & goats probably play a role in transmission of T. evansi to camels and supported that PCR could be used as a diagnostic tool for epidemiological studies on T. evansi in Egypt. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：109 / 114

页数：6

共 29 条

[21] THE ISOENZYME CHARACTERISTICS OF TRYPANOSOMA-EVANSI AND TRYPANOSOMA-EQUIPERDUM ISOLATED FROM DOMESTIC STOCKS IN CHINA [J].