Peptide-based targeting of fluorescent zinc sensors to the plasma membrane of live cells

被引：49

作者：

Radford, Robert J. ^{[1
]}

Chyan, Wen ^{[1
]}

Lippard, Stephen J. ^{[1
]}

机构：

[1] MIT, Dept Chem, Cambridge, MA 02139 USA

来源：

CHEMICAL SCIENCE | 2013年 / 4卷 / 08期

关键词：

ZN2+; PROBE; IONS; COORDINATION; CHEMOSENSORS; PANCREAS; PROTEINS; PROSTATE; ZINQUIN; BIOLOGY;

D O I：

10.1039/c3sc50974e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Combining fluorescent zinc sensors with the facile syntheses and biological targeting capabilities of peptides, we created green- and blue-emitting probes that (i) are readily prepared on the solid-phase, (ii) retain the photophysical and zinc-binding properties of the parent sensor, and (iii) can be directed to the extracellular side of plasma membranes in live cells for detection of mobile zinc.

引用

页码：3080 / 3084

页数：5

共 52 条

[1]

[Anonymous], 2007, Biological Inorganic Chemistry: Structure and Reactivity

[2]

Bourel L, 2000, J PEPT SCI, V6, P264, DOI 10.1002/1099-1387(200006)6:6<264::AID-PSC248>3.3.CO

[3]

2-1

[4] Measuring picomolar intracellular exchangeable zinc in PC-12 cells using a ratiometric fluorescence biosensor [J].