Examining Mechanisms of Pyrethroid Resistance in Eggs of Two Populations of the Chagas' Disease Vector Triatoma infestans (Hemiptera: Reduviidae)

被引:15
作者
Roca-Acevedo, G. [1 ]
Picollo, M. I. [1 ]
Capriotti, N. [2 ]
Sierra, I. [2 ]
Santo-Orihuela, P. L. [1 ]
机构
[1] UNIDEF CONICET, Ctr Invest Plagas & Insecticidas, Buenos Aires, DF, Argentina
[2] Univ Nacl La Plata, Lab Genet & Genom Func, Ctr Reg Estudios Genom, Buenos Aires, DF, Argentina
关键词
Triatoma infestans; resistance; pyrethroid; egg; immature; INSECTICIDE RESISTANCE; SODIUM-CHANNEL; NORTHERN ARGENTINA; GRAN CHACO; BOLIVIA; MUTATIONS; LARVAE;
D O I
10.1093/jme/tjv078
中图分类号
Q96 [昆虫学];
学科分类号
摘要
We found measurable activity of monooxigenases and pyrethroid esterases throughout embryonic development. The pyrethroid esterase activity grew steadily throughout development in all the studied populations and was highest in eggs 12 d old. Mean enzyme activity increased from 13.6 to 16.3 and 22.2 picomol 7-hydroxycoumarin/min (7-OHC) in eggs of 4-, 7-, and 12 d old from the susceptible reference bug colony. Mean activity of resistant populations increased from 16.0 to 25.9 picomol 7-OHC/min in eggs of 4- to 12 d old in Entre Rios population, and from 15.9 to 28.9 picomol 7-OHC/min in Campo Largo population. Molecular analysis of susceptible and resistant developing eggs detected L1014F mutation in both resistant populations, but no L925I mutation was found in any of the studied populations. Higher esterase activity and L1014F presence justify the resistance to pyrethroid throughout developing eggs of both studied T. infestans populations. The description of resistance profiles including resistance mechanisms involved will allow a rational design of campaigns for the control of Chagas disease transmission.
引用
收藏
页码:987 / 992
页数:6
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