Structural organization and classification of cytochrome P450 genes in flax (Linum usitatissimum L.)

被引：32

作者：

Babu, Peram Ravindra ^{[1
]}

Rao, Khareedu Venkateswara ^{[1
]}

Reddy, Vudem Dashavantha ^{[1
]}

机构：

[1] Osmania Univ, Ctr Plant Mol Biol, Hyderabad 500007, Andhra Pradesh, India

来源：

GENE | 2013年 / 513卷 / 01期

关键词：

Cytochrome P450; Flax; Gene amplification; Arabidopsis; FATTY-ACIDS; EXPRESSION; BIOSYNTHESIS; HYDROXYLASE; ALIGNMENT; PLANTS; RICE;

D O I：

10.1016/j.gene.2012.10.040

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Flax CYPome analysis resulted in the identification of 334 putative cytochrome P450 (CYP450) genes in the cultivated flax genome. Classification of flax CYP450 genes based on the sequence similarity with Arabidopsis orthologs and CYP450 nomenclature, revealed 10 clans representing 44 families and 98 subfamilies. CYP80, CYP83, CYP92, CYP702, CYP705, CYP708, CYP728, CYP729, CYP733 and CYP736 families are absent in the flax genome. The subfamily members exhibited conserved sequences, length of exons and phasing of introns. Similarity search of the genomic resources of wild flax species Linum bienne with CYP450 coding sequences of the cultivated flax, revealed the presence of 127 CYP450 gene orthologs, indicating amplification of novel CYP450 genes in the cultivated flax. Seven families CYP73, 74, 75, 76, 77, 84 and 709, coding for enzymes associated with phenylpropanoid/fatty acid metabolism, showed extensive gene amplification in the flax. About 59% of the flax CYP450 genes were present in the EST libraries. (c) 2012 Elsevier B.V. All rights reserved.

引用

页码：156 / 162

页数：7

共 27 条

[1] Phylogenomic analysis of UDP glycosyltransferase 1 multigene family in Linum usitatissimum identified genes with varied expression patterns [J].

Barvkar, Vitthal T. ;

Pardeshi, Varsha C. ;

Kale, Sandip M. ;

Kadoo, Narendra Y. ;

Gupta, Vidya S. .

BMC GENOMICS, 2012, 13 :175

[2] Flaxseed and its components reduce metastasis after surgical excision of solid human breast tumor in nude mice [J].

Chen, JM ;

Wang, L ;

Thompson, LU .

CANCER LETTERS, 2006, 234 (02) :168-175

[3] SSR-based linkage map of flax (Linum usitatissimum L.) and mapping of QTLs underlying fatty acid composition traits [J].

Cloutier, Sylvie ;

Ragupathy, Raja ;

Niu, Zhixia ;

Duguid, Scott .

MOLECULAR BREEDING, 2011, 28 (04) :437-451

[4] Mechanisms and control of rapid genomic changes in flax [J].

Cullis, CA .

ANNALS OF BOTANY, 2005, 95 (01) :201-206

[5]

Cullis C, 2011, WILD CROP RELATIVES: GENOMIC AND BREEDING RESOURCES - OILSEEDS, P177, DOI 10.1007/978-3-642-14871-2_11

[6] MUSCLE: multiple sequence alignment with high accuracy and high throughput [J].

Edgar, RC .

NUCLEIC ACIDS RESEARCH, 2004, 32 (05) :1792-1797

[7] Development and validation of a flax (Linum usitatissimum L.) gene expression oligo microarray [J].

Fenart, Stephane ;

Ndong, Yves-Placide Assoumou ;

Duarte, Jorge ;

Riviere, Nathalie ;

Wilmer, Jeroen ;

van Wuytswinkel, Olivier ;

Lucau, Anca ;

Cariou, Emmanuelle ;

Neutelings, Godfrey ;

Gutierrez, Laurent ;

Chabbert, Brigitte ;

Guillot, Xavier ;

Tavernier, Reynald ;

Hawkins, Simon ;

Thomasset, Brigitte .

BMC GENOMICS, 2010, 11

[8] Evolution of gene order in the genomes of two related yeast species [J].

Fischer, G ;

Neuvéglise, C ;

Durrens, P ;

Gaillardin, C ;

Dujon, B .

GENOME RESEARCH, 2001, 11 (12) :2009-2019

[9] Developing genomic resources in two Linum species via 454 pyrosequencing and genomic reduction [J].

Fu, Yong-Bi ;

Peterson, Gregory W. .

MOLECULAR ECOLOGY RESOURCES, 2012, 12 (03) :492-500

[10] Whole genome co-expression analysis of soybean cytochrome P450 genes identifies nodulation-specific P450 monooxygenases [J].

Guttikonda, Satish K. ;

Trupti, Joshi ;

Bisht, Naveen C. ;

Chen, Hui ;

An, Yong-Qiang C. ;

Pandey, Sona ;

Xu, Dong ;

Yu, Oliver .

BMC PLANT BIOLOGY, 2010, 10

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