CX3CR1+ macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentium

被引：103
作者：
Manta, C. [1 ]
Heupel, E. [2 ]
Radulovic, K. [1 ]
Rossini, V. [1 ]
Garbi, N. [3 ,4 ]
Riedel, C. U. [2 ]
Niess, J. H. [1 ]
机构：
[1] Univ Ulm, Dept Internal Med 1, Ulm, Germany
[2] Univ Ulm, Inst Microbiol & Biotechnol, Ulm, Germany
[3] Inst Mol Med, Dept Mol Immunol, Bonn, Germany
[4] Inst Expt Immunol IMMEI, Bonn, Germany
来源：
MUCOSAL IMMUNOLOGY | 2013年 / 6卷 / 01期
关键词：
INFLAMMATORY-BOWEL-DISEASE; DENDRITIC CELLS; HOST-DEFENSE; INTESTINAL HOMEOSTASIS; FLUORESCENT PROTEIN; CROHNS-DISEASE; FRACTALKINE; IMMUNITY; EXPRESSION; RESPONSES;
D O I：
10.1038/mi.2012.61
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Innate immune cells, such as intestinal epithelial cells, dendritic cells (DCs), macrophages, granulocytes, and innate lymphoid cells provide a first line of defence to enteric pathogens. To study the role of CX(3)CR1(+) DCs and macrophages in host defence, we infected CX(3)CR1-GFP animals with Citrobacter rodentium. When transgenic CX(3)CR1-GFP animals are infected with the natural mouse pathogen C. rodentium, CX(3)CR1(-/-) animals showed a delayed clearance of C. rodentium as compared with (age- and sex-matched) wild-type B6 animals. The delayed clearance of C. rodentium is associated with reduced interleukin (IL)-22 expression. In C. rodentium-infected CX(3)CR1-GFP animals, IL-22 producing lymphoid-tissue inducer cells (LTi cells) were selectively reduced in the absence of CX(3)CR1. The reduced IL-22 expression correlates with decreased expression of the antimicrobial peptides RegIII beta and RegIII gamma The depletion of CX(3)CR1(+) cells by diphtheria toxin injection in CX(3)CR1-GFPxCD11c.DOG animals confirmed the role of CX(3)CR1(+) phagocytes in establishing IL-22 production, supporting the clearance of a C. rodentium infection.
引用
收藏
页码：177 / 188
页数：12
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