1H, 13C and 15N resonance assignments of the GTPase-activating (GAP) and Ral binding domains (GBD) of RLIP76 (RalBP1)

被引：4

作者：

Rajasekar, Karthik V. ^{[1
]}

Campbell, Louise J. ^{[1
]}

Nietlispach, Daniel ^{[1
]}

Owen, Darerca ^{[1
]}

Mott, Helen R. ^{[1
]}

机构：

[1] Univ Cambridge, Dept Biochem, Cambridge CB2 1GA, England

来源：

BIOMOLECULAR NMR ASSIGNMENTS | 2012年 / 6卷 / 02期

基金：

英国医学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

RLIP76; RalBP1; Ral GTPase; Multidomain protein; Endocytosis; Signalling; MULTIDIMENSIONAL NMR; PUTATIVE EFFECTOR; PROTEINS; IDENTIFICATION; HOMOLOGY; COMPLEX; TARGET;

D O I：

10.1007/s12104-011-9337-y

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

RLIP76 (also known as RalBP1) is an effector for Ral small G proteins. RLIP76 is a multifunctional, multi-domain protein that includes a GTPase activating domain for the Rho family (RhoGAP domain) and a GTPase binding domain (GBD) for the Ral small G proteins. The juxtaposition of these two domains (GAP and GBD) may be a strategy employed to co-ordinate regulation of Rho family and Ral-controlled signalling pathways at a crossover node. Here we present the H-1, N-15 and C-13 NMR backbone and sidechain resonance assignments of the GAP and GBD di-domain (31 kDa).

引用

页码：119 / 122

页数：4

共 13 条

[1] Transport of glutathione conjugates and chemotherapeutic drugs by RLIP76 (RALBP1): A novel link between G-protein and tyrosine kinase signaling and drug resistance [J].

Awasthi, S ;

Singhal, SS ;

Sharma, R ;

Zimniak, P ;

Awasthi, YC .

INTERNATIONAL JOURNAL OF CANCER, 2003, 106 (05) :635-646

[2]

CANTOR SB, 1995, MOL CELL BIOL, V15, P4578

[3] The RaIB-RLIP76 Complex Reveals a Novel Mode of RaI-Effector Interaction [J].

Fenwick, R. Brynmor ;

Campbell, Louise J. ;

Rajasekar, Karthik ;

Prasannan, Sunil ;

Nietlispach, Daniel ;

Camonis, Jacques ;

Owen, Darerca ;

Mott, Helen R. .

STRUCTURE, 2010, 18 (08) :985-995

[4] Global folds of highly deuterated, methyl-protonated proteins by multidimensional NMR [J].

Gardner, KH ;

Rosen, MK ;

Kay, LE .

BIOCHEMISTRY, 1997, 36 (06) :1389-1401

[5] The use of 2H, 13C, 15N multidimensional NMR to study the structure and dynamics of proteins [J].

Gardner, KH ;

Kay, LE .

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 1998, 27 :357-406

[6] Identification and characterization of a novel protein interacting with Ral-binding protein 1, a putative effector protein of Ral [J].

Ikeda, M ;

Ishida, O ;

Hinoi, T ;

Kishida, S ;

Kikuchi, A .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (02) :814-821

[7]

Jullien-Flores V, 2000, J CELL SCI, V113, P2837

[8] BRIDGING RAL GTPASE TO RHO-PATHWAYS - RLIP76, A RAL EFFECTOR WITH CDC42/RAC GTPASE-ACTIVATING PROTEIN ACTIVITY [J].

JULLIENFLORES, V ;

DORSEUIL, O ;

ROMERO, F ;

LETOURNEUR, F ;

SARAGOSTI, S ;

BERGER, R ;

TAVITIAN, A ;

GACON, G ;

CAMONIS, JH .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (38) :22473-22477

[9]

Park SH, 1995, ONCOGENE, V11, P2349

[10] Ral: Mediator of membrane trafficking [J].

van Dam, Ellen M. ;

Robinson, Phillip J. .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2006, 38 (11) :1841-1847

← 1 2 →