Implication of C-type natriuretic peptide-3 signaling in glycosaminoglycan synthesis and chondrocyte hypertrophy during TGF-β1 induced chondrogenic differentiation of chicken bone marrow-derived mesenchymal stem cells

被引：24

作者：

Kocamaz, Erdogan ^{[1
]}

Gok, Duygu ^{[1
]}

Cetinkaya, Ayse ^{[1
]}

Tufan, A. Cevik ^{[1
]}

机构：

[1] Pamukkale Univ, Dept Histol & Embryol, Sch Med, Denizli, Turkey

来源：

JOURNAL OF MOLECULAR HISTOLOGY | 2012年 / 43卷 / 05期

关键词：

CNP; NPR-B; Chondrogenesis; Endochondral ossification; Glycosaminoglycan synthesis; Chondrogenic hypertrophy; HUMAN TRABECULAR BONE; ALTERED N-CADHERIN; RECEPTOR-B; MUTATIONS; COLLAGEN; GROWTH;

D O I：

10.1007/s10735-012-9430-2

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

This study investigated the involvement of CNP-3, chick homologue for human C-type natriuretic peptide (CNP), in TGF-beta 1 induced chondrogenic differentiation of chicken bone marrow-derived mesenchymal stem cells (MSCs). Chondrogenic differentiation of MSCs in pellet cultures was induced by TGF-beta 1. Chondrogenic differentiation and glycosaminoglycan synthesis were analyzed on the basis of basic histology, collagen type II expression, and Alcian blue staining. Antibodies against CNP and NPR-B were used to block their function during these processes. Results revealed that expression of CNP-3 and NPR-B in MSCs were regulated by TGF-beta 1 in monolayer cultures at mRNA level. In pellet cultures of MSCs, TGF-beta 1 successfully induced chondrogenic differentiation and glycosaminoglycan synthesis. Addition of CNP into the TGF-beta 1 supplemented chondrogenic differentiation medium further induced the glycosaminoglycan synthesis and hypertrophy of differentiated chondrocytes in these pellets. Pellets induced with TGF-beta 1 and treated with antibodies against CNP and NPR-B, did show collagen type II expression, however, Alcian blue staining showing glycosaminoglycan synthesis was significantly suppressed. In conclusion, CNP-3/NPR-B signaling may strongly be involved in synthesis of glycosaminoglycans of the chondrogenic matrix and hypertrophy of differentiated chondrocytes during TGF-beta 1 induced chondrogenic differentiation of MSCs.

引用

页码：497 / 508

页数：12

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