Comparison of three decontamination methods for Mycobacterium bovis isolation

被引：27

作者：

Ambrosio, Simone Rodrigues ^{[1
]}

de Deus Oliveira, Eugenia Marcia ^{[2
]}

Rosales Rodriguez, Cesar Alejandro ^{[1
]}

Ferreira Neto, Jose Soares ^{[1
]}

Amaku, Marcos ^{[1
]}

机构：

[1] Univ Sao Paulo, Dept Med Vet Prevent & Saude Anim, Fac Med Vet & Zootecn, BR-05508270 Sao Paulo, Brazil

[2] Univ Fed Bahia, Salvador, BA, Brazil

来源：

BRAZILIAN JOURNAL OF MICROBIOLOGY | 2008年 / 39卷 / 02期

关键词：

Mycobacterium bovis; decontamination; petroff; HPC;

D O I：

10.1590/S1517-83822008000200008

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sixty samples of tissue fragments with lesions suggestive of tuberculosis from bovine abattoirs, kept in saturated solution of sodium borate, were subjected to four treatments: 4% NaOH (Petroff Method), 12% H2SO4 and 1.5% HPC (1-Hexadecylpyridinium Chloride) decontamination, and physiological saline solution (control). The HPC method showed the lowest contamination rate (3%) when compared to control (88%, p<0.001), NaOH (33%, p<0.001) and H2SO4 (21.7%, p<0.002). Regarding the isolation success, the HPC method was better (40%) than the control (3%, p<0.001), NaOH (13%, p=0.001) and H2SO4 (1.7%, p<0.001) methods. These results indicate that HPC is an alternative to the Petroff method.

引用

页码：241 / 244

页数：4

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