Implementing homo- and heterodecoupling in region-selective HSQMBC experiments

被引：34

作者：

Castanar, Laura

Sauri, Josep

Nolis, Pau

Virgili, Albert

Parella, Teodor ^{[1
]}

机构：

[1] Univ Autonoma Barcelona, Serv Ressonancia Magnet Nucl, E-08193 Bellaterra, Catalonia, Spain

来源：

JOURNAL OF MAGNETIC RESONANCE | 2014年 / 238卷

关键词：

Pure-shift; Band-selective HSQMBC; Proton-carbon coupling constants; Homonuclear decoupling; Sensitivity improvement; Resolution enhancement; HETERONUCLEAR COUPLING-CONSTANTS; RESIDUAL DIPOLAR COUPLINGS; BROAD-BAND; NATURAL-ABUNDANCE; NMR-SPECTROSCOPY; SPECTRA; RESOLUTION; HSQC; H-1; SENSITIVITY;

D O I：

10.1016/j.jmr.2013.10.022

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An NMR method to enhance the sensitivity and resolution in band-selective long-range heteronuclear correlation spectra is proposed. The excellent in-phase nature of the seIHSQMBC experiment allows that homonuclear and/or heteronuclear decoupling can be achieved in the detected dimension of a 2D multiple-bond correlation map, obtaining simplified cross-peaks without their characteristic fine J multiplet structure. The experimental result is a resolution improvement while the highest sensitivity is also achieved. Specifically, it is shown that the H-1-homodecoupled band-selective (HOBS) HSQMBC experiment represents a new way to measure heteronuclear coupling constants from the simplified in-phase doublets generated along the detected dimension. (C) 2013 Elsevier Inc. All rights reserved.

引用

收藏

页码：63 / 69

页数：7

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