Taxonomic evaluation of the genus Enterobacter based on multilocus sequence analysis (MLSA): Proposal to reclassify E. nimipressuralis and E. amnigenus into Lelliottia gen. nov as Lelliottia nimipressuralis comb. nov and Lelliottia amnigena comb. nov., respectively, E. gergoviae and E. pyrinus into Pluralibacter gen. nov as Pluralibacter gergoviae comb. nov and Pluralibacter pyrinus comb. nov., respectively, E. cowanii, E. radicincitans, E. oryzae and E. arachidis into Kosakonia gen. nov as Kosakonia cowanii comb. nov., Kosakonia radicincitans comb. nov., Kosakonia oryzae comb. nov and Kosakonia arachidis comb. nov., respectively, and E. turicensis, E. helveticus and E. pulveris into Cronobacter as Cronobacter zurichensis nom. nov., Cronobacter helveticus comb. nov and Cronobacter pulveris comb. nov., respectively, and emended description of the genera Enterobacter and Cronobacter

被引：286
作者：
Brady, Carrie [1 ]
Cleenwerck, Ilse [2 ]
Venter, Stephanus [3 ]
Coutinho, Teresa [3 ]
De Vos, Paul [2 ]
机构：
[1] Univ Ghent, Fac Sci, Microbiol Lab, LM UGent, B-9000 Ghent, Belgium
[2] Univ Ghent, Fac Sci, BCCM LMG Bacteria Collect, B-9000 Ghent, Belgium
[3] Univ Pretoria, Dept Microbiol & Plant Pathol, FABI, ZA-0002 Pretoria, South Africa
来源：
SYSTEMATIC AND APPLIED MICROBIOLOGY | 2013年 / 36卷 / 05期
关键词：
Enterobacter; MLSA; Taxonomy; Cronobacter; Enterobacteriaceae; CLINICAL SPECIMENS; PHYLOGENETIC TREE; TAYLORAE FARMER; ZUMOFF; 1988; 1966; DICKEY; DNA; IDENTIFICATION; HYBRIDIZATION; REASSIGNMENT; RELATEDNESS;
D O I：
10.1016/j.syapm.2013.03.005
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The taxonomy of Enterobacter has a complicated history, with several species transferred to and from this genus. Classification of strains is difficult owing to its polyphyletic nature, based on 16S rRNA gene sequences. It has been previously acknowledged that Enterobacter contains species which should be transferred to other genera. In an attempt to resolve the taxonomy of Enterobacter, MLSA based on partial sequencing of protein-encoding genes (gyrB, rpoB, infB and atpD) was performed on the type strains and reference strains of Enterobacter, Cronobacter and Serratia species, as well as members of the closely related genera Citrobacter, Klebsiella, Kluyvera, Leclercia, Mangrovibacter, Raoultella and Yokenella. Phylogenetic analyses of the concatenated nucleotide sequences revealed that Enterobacter can be divided into five strongly supported MLSA groups, suggesting that the species should be reclassified into five different genera. Further support for this was provided by a concatenated amino acid tree, phenotypic characteristics and fatty acid profiles, enabling differentiation of the MLSA groups. Three novel genera are proposed: Lelliottia gen. nov., Pluralibacter gen. nov. and Kosakonia gen. nov. and the following new combinations: Lelliottia nimipressuralis comb. nov., Lelliottia amnigena comb. nov., Pluralibacter gergoviae comb. nov., Pluralibacter pyrinus comb. nov., Kosakonia cowanii comb. nov., Kosakonia radicincitans comb. nov., Kosakonia oryzae comb. nov., Kosakonia arachidis comb. nov., Cronobacter helveticus comb. nov. and Cronobacter pulveris comb. nov. Additionally, the novel epithet Cronobacter zurichensis nom. nov. is proposed for the reclassification of Enterobacter turicensis into the genus Cronobacter, as Cronobacter turicensis (Iversen et al., 2008) is already in use. (C) 2013 Elsevier GmbH. All rights reserved.
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页码：309 / 319
页数：11
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