Asteraceae Artemisia campestris and Artemisia herba-alba Essential Oils Trigger Apoptosis and Cell Cycle Arrest in Leishmania infantum Promastigotes

被引：32

作者：

Aloui, Zohra ^{[1
]}

Messaoud, Chokri ^{[2
]}

Haoues, Meriam ^{[3
]}

Neffati, Noura ^{[1
]}

Jamoussi, Imen Bassoumi ^{[1
]}

Essafi-Benkhadir, Khadija ^{[1
]}

Boussaid, Mohamed ^{[2
]}

Guizani, Ikram ^{[1
]}

Karoui, Habib ^{[1
]}

机构：

[1] Univ Tunis El Manar, Lab Epidemiol Mol & Pathol Expt Appl Malad Infect, Inst Pasteur Tunis, 13 Pl Pasteur,BP 74, Tunis 1002, Tunisia

[2] INSAT, Unite Ressources Phytogenet & Biotechnol Vegetale, BP 676, Tunis 1080, Tunisia

[3] Univ Tunis El Manar, Inst Pasteur Tunis, Lab Rech Transmiss Controle & Immunobiol Infect L, 13 Pl Pasteur,BP 74, Tunis 1002, Tunisia

来源：

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2016年 / 2016卷

关键词：

IN-VITRO; ANTILEISHMANIAL ACTIVITY; DONOVANI PROMASTIGOTES; DEATH; DIFFERENTIATION; ABSINTHIUM; TOXICITY; PRODUCTS; EXTRACTS; VACCINE;

D O I：

10.1155/2016/9147096

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

We report the chemical composition and anti-Leishmania and antioxidant activity of Artemisia campestris L. and Artemisia herba-alba Asso. essential oils ( EOs). Our results showed that these extracts exhibit different antioxidant activities according to the used assay. The radical scavenging effects determined by DPPH assay were of IC50 = 3.3mg/mL and IC50 = 9.1mg/mL for Artemisia campestris and Artemisia herba-alba essential oils, respectively. However, antioxidant effects of both essential oils, determined by ferric-reducing antioxidant power ( FRAP) assay, were in the same range ( 2.3 and 2.97 mg eq EDTA/g EO, resp.), while the Artemisia herba-alba essential oil showed highest chelating activity of Fe2+ ions ( 27.48mM Fe2+). Interestingly, we showed that both EOs possess dose-dependent activity against Leishmania infantum promastigotes with IC50 values of 68 mu g/mL and 44 mu g/mL for A. herba-alba and A. campestris, respectively. We reported, for the first time, that antileishmanial activity of both EOs was mediated by cell apoptosis induction and cell cycle arrest at the sub-G0/G1 phase. All our results showed that EOs from A. herba-alba and A. campestris plants are promising candidates as anti-Leishmania medicinal products.

引用

页数：15

共 58 条

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